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US 2021/0009934 A1Published application
Cell culture device
AssigneesDai Nippon Printing Co., Ltd., Osaka University, Terumo Kabushiki Kaisha
InventorsTaro NAGAI, Yoshiki SAWA, Shigeru MIYAGAWA, Kenji OYAMA
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Abstract
When detachment of a cell sheet from a culture dish has started at an unintended timing, immediately detach the cell sheet from the culture dish while preventing generation of wrinkles and the like in the cell sheet. A cell culture device captures an image of cells in the culture dish, and controls a cooling mechanism and a shaking mechanism based on the image so as to shake the cells while cooling them.
Claims9
A cell culture device for culturing cells, comprising:
- a plate adapted to have placed thereon a culture dish for holding cells;
- a cooling mechanism that cools the cells held by the culture dish;
- a shaking mechanism that shakes the culture dish or the plate;
- an imaging unit that captures an image of the cells held by the culture dish;
- and
- a controller that operates the cooling mechanism and the shaking mechanism,
- wherein the controller operates the cooling mechanism and the shaking mechanism according to a result of determination of whether to operate the cooling mechanism and the shaking mechanism based on the image of the cells captured by the imaging unit,
- wherein the controller determines if at least a part of the cells has been detached from the culture dish by analyzing shapes of the cells based on the image, and
- wherein the controller, upon determining that at least a part of the cells has been detached from the culture dish, operates the cooling mechanism and the shaking mechanism.
6 more elements
(canceled)
A method for collecting cells comprising:
- capturing an image of the cells cultivated on a culture dish and analyzing a shape of the cells according to the captured image, thereby determining whether at least a part of the cells has been detached from the culture dish;
- and
- upon determining that at least a part of the cells has been detached from the culture dish, performing cooling and shaking operation to collect the cells.
Dependencies are inferred from the claim text.