Methods and systems for separating biological particles

A microfluidic device, comprising:

• a fluidic channel;
• and
• an array of obstacles disposed in said fluidic channel, and oriented at an angle greater than 0° relative to a direction of a fluid flow in said fluidic channel,
• wherein an obstacle of said array of obstacles is oriented at an angle of less than 90° with respect to a surface of said obstacles, and wherein said array of obstacles is configured to separate one or more target analytes from a fluid flowing through said fluidic channel.

A microfluidic device, comprising:

• a fluidic channel;
• and
• an array of obstacles disposed in said fluidic channel;
• wherein said array of obstacles is configured to separate one or more senescent cells from a fluid having a volume less than or equal to about 1 milliliter (mL) at an efficiency greater than about 70% upon flow of said fluid through said fluidic channel.

A method, comprising:

• (a) directing a fluid comprising one or more target analytes into a microfluidic device, said microfluidic device comprising:
• a fluidic channel;
• and
• an array of obstacles disposed in said fluidic channel, wherein said array of obstacles is oriented at an angle greater than 0° relative to a direction of a fluid flow in said fluidic channel;
• (b) directing said fluid to flow through said fluidic channel;
• and
• (c) separating at least a portion of said one or more target analytes from said fluid using said array of obstacles upon flow of said fluid through said fluidic channel.