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AssigneePaul Scherrer Institut
InventorsGABRIEL AEPPLI, JOE BAILEY, PAUL DALBY, JOHN HALES, GUY MATMON
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Selective amplification of DNA in PCR exponentially increases the signal in molecular diagnostics for nucleic acids, but analogous techniques exist for signal enhancement in clinical tests for proteins or cells. Instead, the signal from affinity-based measurements of these biomolecules depends linearly on probe concentration. Substituting antibody-based probes tagged for fluorescent quantification with lasing detection probes would create a new platform for biomarker quantification based on optical rather than enzymatic amplification. Here, a viral laser is constructed which bridges synthetic biology and laser physics, and demonstrates viral-lasing probes for biosensing. At the transition to lasing, photon flux from the probes increases by five orders of magnitude and narrows spectral linewidth to below 5.0 nm. Viral-lasing probes display an unprecedented >1,000,000% increase in signal from only a 50% increase in probe concentration, using fluorimeter-compatible optics, and can detect biomolecules at clinically-relevant concentrations.
1-9. (canceled)
A method for biosensing a binding ability among a biomolecule, an antibody or a membrane protein, and a virus, the method comprising the following steps:
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