Methods and compositions for controlling plant viral infection
AssigneeMonsanto Technology LLC
InventorCharles HAGEN
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Abstract
Methods for treatment and prevention of Potexvirus disease in plants are provided. Further provided are compositions for treatment or prevention of Potexvirus disease in plants, and methods for reducing expression of a Potexvirus gene and for identifying polynucleotides useful in modulating gene expression in plant viruses.
Claims22
A method of treatment or prevention of a Pepino Mosaic Virus (PepMV) infection in a plant, the method comprising topically applying to said plant a composition comprising a double-stranded RNA polynucleotide and a transfer agent, wherein the double-stranded RNA polynucleotide is complementary to all or a portion of an essential PepMV gene sequence or an RNA transcript thereof, wherein the essential PepMV gene is from the PepMV genome 5′ region, is the PepMV RNA-dependent RNA polymerase (RdRp) gene or is the PepMV capsid protein (CP) gene and wherein the symptoms of viral infection or development of symptoms are reduced or eliminated in said plant relative to a plant not treated with said composition when grown under the same conditions.
7-8. (canceled)
A composition comprising a double-stranded RNA polynucleotide and a transfer agent, wherein said double-stranded RNA polynucleotide is complementary to all or a portion of an essential PepMV gene sequence or an RNA transcript thereof, wherein the essential PepMV gene is from the PepMV genome 5′ region, is the PepMV RNA-dependent RNA polymerase (RdRp) gene or is the PepMV capsid protein (CP) gene, and wherein topical application of the composition to a plant reduces or eliminates the symptoms of PepMV infection or development in said plant relative to a plant not treated with said composition when grown under the same conditions.
11-12. (canceled)
A method of identifying double-stranded RNA polynucleotides useful in modulating PepMV gene expression when topically treating a plant comprising:
- a) providing a plurality of double-stranded RNA polynucleotides that comprise a region complementary to all or a part of an essential PepMV gene or RNA transcript thereof;
- b) topically treating said plant with one or more of said double-stranded RNA polynucleotides and a transfer agent;
- c) analyzing said plant or extract for modulation of symptoms of PepMV infection;
- and
- d) selecting a double-stranded RNA polynucleotide capable of modulating the symptoms or occurrence of PepMV infection.
A method of treatment or prevention of a PepMV infection in a plant comprising:
- topically applying to said plant a composition comprising an antisense single-stranded DNA polynucleotide and a transfer agent, wherein said antisense single-stranded DNA polynucleotide is complementary to all or a portion of an essential PepMV gene sequence or an RNA transcript thereof, wherein the essential PepMV gene is from the PepMV genome 5′ region, is the PepMV RNA-dependent RNA polymerase (RdRp) gene or is the PepMV capsid protein (CP) gene and wherein the symptoms of viral infection or development of symptoms are reduced or eliminated in said plant relative to a plant not treated with said composition when grown under the same conditions.
Dependencies are inferred from the claim text.