# US 2020/0401081 A1 · Coherent Optical Holographic Imaging > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2020/0401081 A1 - Document ID: US20200401081A1 - Status: Published application - Kind: A1 - Application number: 16/906,354 - Filed: 2020-06-19 - Published: 2020-12-24 - Earliest claimed: 2019-06-19 - Assignees: The Johns Hopkins University - Applicants: The Johns Hopkins University - Inventors: David W. Blodgett; Carissa L. Rodriguez; Austen T. Lefebvre; Eyal Bar-Kochba - Web page: https://patentgrep.com/patent/US20200401081A1 ## CPC - G03H 1/2205: {using downstream optical component} - A61B 5/0066: {Optical coherence imaging} - G01N 15/147: {the analysis being performed on a sample stream} - G02B 13/22: Telecentric objectives or lens systems - G02B 21/365: {Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence)} - G02B 27/1006: {for splitting or combining different wavelengths (G02B27/1086, G02B27/141 take precedence)} - G03H 1/0443: {Digital holography, i.e. recording holograms with digital recording means (holobject computation G03H1/0866)} - G03H 1/0866: {Digital holographic imaging, i.e. synthesizing holobjects from holograms} - A61B 5/0261: {using optical means, e.g. infrared light} - G01N 2015/1006: {for cytology} - G03H 2001/0463: {Frequency heterodyne, i.e. one beam is frequency shifted} - G03H 2001/2207: {Spatial filter, e.g. for suppressing higher diffraction orders} - G03H 2210/62: Moving object - G03H 2222/20: Coherence of the light source - G03H 2222/45: Interference beam at recording stage, i.e. following combination of object and reference beams - G03H 2226/02: Computing or processing means, e.g. digital signal processor [DSP] - G03H: HOLOGRAPHIC PROCESSES OR APPARATUS (holograms, e.g. point holograms, used as ordinary optical elements G02B5/32; producing stereoscopic or other three-dimensional effects G02B30/00; diffraction-grating systems G02B27/44; systems using moiré fringes G02B27/60; optical logic elements G02F3/00; stereo-photography G03B35/00; photosensitive materials or processes for photographic purposes G03C; {stereo-photographic or similar processes G03C9/00}; apparatus for processing exposed photographic materials G03D; analogue computers performing mathematical operations with the aid of optical elements G06E3/00; authentication by radiation, of concealed information carried by holograms or diffraction gratings G06K19/16; holographic storage G11B7/0065, G11C13/04; {stereoscopic or other three dimensional effects in television systems H04N13/00}) - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS ## Abstract A holographic imaging system may include an optical source configured to output a source beam, a splitter configured to split the source beam into a reference beam and an object beam that is incident on a target to form a scattered object beam, and a pre-filter comprising a telecentric lens and a spatial filter. The pre-filter may be configured to receive the scattered object beam and filter diffuse light from the scattered object beam to form a filtered scattered object beam. The system may also include a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam, and an imaging array configured to receive the interference beam and generate raw holographic data based on the interference beam. ## Claims ### Claim 1 A holographic imaging system comprising: an optical source configured to output a source beam; a splitter configured to split the source beam into a reference beam and an object beam, the object beam being incident on a target to form a scattered object beam; a pre-filter comprising a telecentric lens and a spatial filter, the pre-filter being configured to receive the scattered object beam and filter diffuse light from the scattered object beam to form a filtered scattered object beam; a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam; and an imaging array configured to receive the interference beam and generate raw holographic data based on the interference beam. ### Claim 2 (depends on 1) The holographic imaging system of claim 1, wherein the telecentric lens in configured to magnify the scattered object beam and apply the magnified scattered object beam to the spatial filter to perform filtering to form the filtered scattered object beam. ### Claim 3 (depends on 1) The holographic imaging system of claim 1, wherein the telecentric lens and the spatial filter are configured to filter light from the scattered object beam having an incident angle greater than a maximum coherent mixing angle for the holographic imaging system. ### Claim 4 (depends on 1) The holographic imaging system of claim 1 further comprising an array controller operably coupled to the imaging array to control operation of the imaging array; wherein the array controller is configured to control the imaging array to perform a series of frame capture events at a controlled frame rate; wherein, for each frame capture event, measurements of the interference beam are taken and converted into a frame of raw holographic data; and wherein a frame rate period based on the controlled frame rate is shorter than a decorrelation time of the target. ### Claim 5 (depends on 4) The holographic imaging system of claim 4, wherein the controlled frame rate is greater than 1000 Hertz. ### Claim 6 (depends on 1) The holographic imaging system of claim 1, further comprising a reference beam splitter configured to split a time delayed reference beam from the reference beam; wherein the imaging array is configured to receive the interference beam associated with the reference beam at a first time during a single integration time of the imaging array and configured to receive a time delayed interference beam associated with the time delayed reference beam during the single integration time of the imaging array. ### Claim 7 (depends on 6) The holographic imaging system of claim 6, wherein an incident angle of the interference beam on the imaging array is different from an incident angle of the time delayed interference beam on the imaging array. ### Claim 8 (depends on 7) The holographic imaging system of claim 7, wherein raw holographic data associated with the interference beam is differentiated from raw holographic data associated with the time delayed interference beam based on the difference in the incident angle of the interference beam on the imaging array from an incident angle of the time delayed interference beam on the imaging array. ### Claim 9 (depends on 1) The holographic imaging system of claim 1, wherein the optical source is configured to output a source beam at a wavelength that causes the target to provide at least a threshold scatter level in the scattered object beam. ### Claim 10 A method comprising: outputting a source beam from an optical source; splitting the source beam into a reference beam and an object beam, the object beam being incident on a target to form a scattered object beam; receiving the scattered object beam and filtering diffuse light from the scattered object beam via a telecentric lens and a spatial filter to form a filtered scattered object beam; combining the filtered scattered object beam with the reference beam to form an interference beam; and receiving the interference beam at an imaging array. ### Claim 11 (depends on 10) The method of claim 10, further comprising magnifying the scattered object beam via the telecentric lens and applying the magnified scattered object beam to the spatial filter to perform filtering to form the filtered scattered object beam. ### Claim 12 (depends on 10) The method of claim 10, further comprising filtering light from the scattered object beam, via the telecentric lens and the spatial filter, having an incident angle greater than a maximum coherent mixing angle for a holographic imaging system. ### Claim 13 (depends on 10) The method of claim 10 further comprising controlling the imaging array to perform a series of frame capture events at a controlled frame rate; wherein, for each frame capture event, the method further comprises capturing measurements of the interference beam and converting the measurements into a frame of raw holographic data at a time of the frame capture event; and wherein a frame rate period based on the controlled frame rate is shorter than a decorrelation time of the target. ### Claim 14 (depends on 13) The method of claim 13, wherein the controlled frame rate is greater than 1000 Hertz. ### Claim 15 (depends on 10) The method of claim 10, further comprising: splitting a time delayed reference beam from the reference beam; and receiving, at the imaging array, the interference beam associated with the reference beam at a first time during a single integration time of the imaging array and receiving a time delayed interference beam associated with the time delayed reference beam during the single integration time of the imaging array. ### Claim 16 (depends on 15) The method of claim 15 wherein an incident angle of the interference beam on the imaging array is different from an incident angle of the time delayed interference beam on the imaging array. ### Claim 17 (depends on 16) The method of claim 16, wherein raw holographic data associated with the interference beam is differentiated from raw holographic data associated with the time delayed interference beam based on the difference in the incident angle of the interference beam on the imaging array from an incident angle of the time delayed interference beam on the imaging array. ### Claim 18 (depends on 10) The method of claim 10, wherein outputting the source beam comprises outputting the source beam at a wavelength that causes the target to provide at least a threshold scatter level in the scattered object beam. ### Claim 19 An apparatus comprising: a pre-filter comprising a telecentric lens and a spatial filter, the pre-filter being configured to receive a scattered object beam from an interaction with a target and filter diffuse light from the scattered object beam to form a filtered scattered object beam; a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam; and an imaging array configured to receive the interference beam and generate raw holographic data based on the interference beam. ### Claim 20 (depends on 19) The apparatus of claim 19, further comprising an array controller operably coupled to the imaging array to control operation of the imaging array; wherein the array controller is configured to control the imaging array to perform a series of frame capture events at a controlled frame rate; wherein, for each frame capture event, measurements of the interference beam are taken and converted into a frame of raw holographic data; and wherein a frame rate period based on the controlled frame rate is shorter than a decorrelation time of the target.