Biomarkers and Methods for Measuring and Monitoring Inflammatory Disease Activity
AssigneesMeso Scale Technologies, LLC., Crescendo Bioscience, Inc., Oklahoma Medical Research Foundation
InventorsPankaj Oberoi, Steven C. Hoffmann, Guy L. Cavet, Yijing Shen, Nicholas Knowlton, Michael Centola
Life of this patent
Estimating the term…
Abstract
Biomarkers useful for diagnosing and assessing inflammatory disease are provided, along with kits for measuring their expression. The invention also provides predictive models, based on the biomarkers, as well as computer systems, and software embodiments of the models for scoring and optionally classifying samples. The biomarkers include at least two biomarkers selected from the DAIMRK group and the score is a disease activity index (DAI).
Claims30
-231. (canceled)
A method for generating protein level data for a first subject comprising:
- performing at least one immunoassay on a first blood sample from the first subject to generate a first dataset comprising protein level data for at least four protein markers, wherein the at least four protein markers comprise at least four markers selected from chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1);
- C-reactive protein, pentraxin-related (CRP);
- epidermal growth factor (beta-urogastrone) (EGF);
- interleukin 6 (interferon, beta 2) (IL6);
- leptin (LEP);
- matrix metallopeptidase 1 (interstitial collagenase) (MMP1);
- matrix metallopeptidase 3 (stromelysin 1, progelatinase) (MMP3);
- resistin (RETN);
- serum amyloid A1 (SAA1);
- tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A);
- vascular cell adhesion molecule 1 (VCAM1);
- or, vascular endothelial growth factor A (VEGFA);
- and
- wherein the first subject has rheumatoid arthritis (RA) or is suspected of having RA.
A method for generating a protein level score comprising:
- performing at least one immunoassay on a first blood sample from a first subject previously diagnosed with rheumatoid arthritis (RA), or suspected of having RA, to generate protein level data for each protein marker of a plurality of protein markers, wherein the plurality of protein markers comprises a plurality of test markers comprising at least four markers selected from chitinase 3-like 1 (cartilage glycoprotein-39) (CHI3L1);
- C-reactive protein, pentraxin-related (CRP);
- epidermal growth factor (beta-urogastrone) (EGF);
- interleukin 6 (interferon, beta 2) (IL6);
- leptin (LEP);
- matrix metallopeptidase 1 (interstitial collagenase) (MMP1);
- matrix metallopeptidase 3 (stromelysin 1, progelatinase) (MMP3);
- resistin (RETN);
- serum amyloid A1 (SAA1);
- tumor necrosis factor receptor superfamily, member 1A (TNFRSF1A);
- vascular cell adhesion molecule 1 (VCAM1);
- or, vascular endothelial growth factor A (VEGFA);
- and
- combining the protein level data for each test marker to generate the protein level score.
Dependencies are inferred from the claim text.