# US 2020/0399721 A1 · Microbacterium sp. strain and method for producing psicose by using same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2020/0399721 A1 - Document ID: US20200399721A1 - Status: Published application - Kind: A1 - Application number: 15/775,491 - Filed: 2016-12-01 - Published: 2020-12-24 - Earliest claimed: 2015-12-07 - Assignees: Samyang Corporation - Applicants: Samyang Corporation - Inventors: Eun Jin HAN; Sang-Hee LEE; Soun Gyu KWON; Bu-Soo PARK; Sin Hye AHN; Chong Jin PARK; Kang Pyo LEE - Web page: https://patentgrep.com/patent/US20200399721A1 ## CPC - C12N 1/205: Bacterial isolates - C12N 1/20: Bacteria; Culture media therefor - C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60) - C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present invention relates to a newly isolated bacterium belonging to the genus Microbacterium, a composition for producing psicose comprising the strain, and a method for producing psicose using the same. ## Claims ### Claim 1 A method for producing a psicose from a fructose-containing substrate using a bacterium belonging to the genus Microbacterium having a psicose conversion activity to produce a psicose from a fructose. ### Claim 2 (depends on 1) The method for producing a psicose of claim 1, wherein the bacterium belonging to genus Microbacterium is one or higher selected from the group consisting of a microbial cell of the bacterium, a culture of the bacterium, a culture supernatant of the bacterium, an culture extract of the bacterium, and a lysate of the bacterium. ### Claim 3 (depends on 1) The method for producing a psicose of claim 1, comprising a step of culturing Microbacterium genus strain in a medium containing a fructose. ### Claim 4 (depends on 1) The method for producing a psicose of claim 1, wherein a step of reacting the Microbacterium genus strain with the fructose is performed by mixing one or higher selected from the group consisting of a microbial cell of the Microbacterium genus strain, a culture of the strain and a lysate of the strain with the fructose. ### Claim 5 (depends on 4) The method for producing a psicose of claim 4, wherein the step of reacting a bacterium belonging to the genus Microbacterium with the fructose is performed by contacting the fructose to a support immobilized with one or higher selected from the group consisting of a microbial cell of the bacterium belonging to the genus Microbacterium, a culture of the strain and a lysate of the strain. ### Claim 6 (depends on 4) The method for producing a psicose of claim 4, wherein the method further comprises a step of adding one or higher kinds of metal ions selected from the group consisting of manganese and cobalt. ### Claim 7 (depends on 1) The method for producing a psicose of claim 1, wherein the method is characterized by not using a buffer solution. ### Claim 8 (depends on 1) The method for producing a psicose of claim 1, wherein the fructose-containing substrate contains the fructose of a concentration of 40 to 75% (w/w). ### Claim 9 (depends on 1) The method for producing a psicose of claim 1, wherein the method is performed under the condition of pH 6.5 to 9.0 and 40 to 80° C. ### Claim 10 (depends on 1) The method for producing a psicose of claim 1, wherein the bacterium belonging to the genus Microbacterium is Microbacterium foliorum, Microbacterium oxydans, Microbacterium maritypicum, Microbacterium liquefaciens, Microbacterium luteolum, Microbacterium ginsengiterrae, Microbacterium keratanolyticum, Microbacterium natoriense, Microbacterium lacticum, Microbacterium xylanilyticum, Microbacterium koreense, Microbacterium imperial or Microbacterium phyllosphaerae. ### Claim 11 (depends on 1) The method for producing a psicose of claim 1, wherein the bacterium belonging to the genus Microbacterium is Microbacterium foliorum strain deposited as an accession number KCCM11774P. ### Claim 12 A composition for producing a psicose from a fructose, comprising a bacterium belonging to the genus Microbacterium having a psicose conversion activity to produce a psicose from a fructose. ### Claim 13 (depends on 12) The composition of claim 12, wherein the bacterium belonging to the genus Microbacterium has a psicose conversion activity at 40 to 80° C. ### Claim 14 (depends on 12) The composition of claim 12, wherein the bacterium belonging to the genus Microbacterium has a psicose conversion activity at pH 6.5 to 9.0. ### Claim 15 (depends on 12) The composition for producing a psicose of claim 12, wherein the bacterium belonging to the genus Microbacterium has 1.2 to 2.3 times increased psicose conversion activity in the presence of one or higher kinds of metal ions selected from the group consisting of manganese and cobalt, compared to the case without the metal ion. ### Claim 16 (depends on 12) The composition for producing a psicose of claim 12, wherein the bacterium belonging to the genus Microbacterium is Microbacterium foliorum, Microbacterium oxydans, Microbacterium maritypicum, Microbacterium liquefaciens, Microbacterium luteolum, Microbacterium ginsengiterrae, Microbacterium keratanolyticum, Microbacterium natoriense, Microbacterium lacticum, Microbacterium xylanilyticum, Microbacterium koreense, Microbacterium imperial or Microbacterium phyllosphaerae. ### Claim 17 (depends on 12) The composition for producing a psicose of claim 12, wherein the bacterium belonging to the genus Microbacterium deposited as an accession number KCCM11774P. ### Claim 18 (depends on 12) The composition for producing a psicose of claim 12, wherein the bacterium belonging to the genus Microbacterium is one or higher selected from the group consisting of a microbial cell of the strain, a culture of the strain and a lysate of the strain. ### Claim 19 A Microbacterium foliorum, Microbacterium oxydans, Microbacterium maritypicum, Microbacterium liquefaciens, Microbacterium luteolum, Microbacterium ginsengiterrae, Microbacterium keratanolyticum, Microbacterium natoriense, Microbacterium lacticum, Microbacterium xylanilyticum, Microbacterium koreense, Microbacterium imperial or Microbacterium phyllosphaerae strain having an activity to convert a fructose to a psicose. ### Claim 20 (depends on 19) The strain of claim 19, wherein the bacterium belonging to the genus Microbacterium is Microbacterium foliorum strain deposited as an accession number KCCM11774P.