# US 2020/0399714 A1 · Cancer-related biological materials in microvesicles

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- Number: US 2020/0399714 A1
- Document ID: US20200399714A1
- Status: Published application
- Kind: A1
- Application number: 17/014,540
- Filed: 2020-09-08
- Published: 2020-12-24
- Earliest claimed: 2010-08-31
- Assignees: The General Hospital Corporation
- Applicants: The General Hospital Corporation
- Inventors: Johan Karl Olov SKOG; Leonora BALAJ; Mikkel NOERHOLM; Xandra BREAKEFIELD
- Web page: https://patentgrep.com/patent/US20200399714A1

## CPC

- C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575)
- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- C12Q 2600/106: Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
- C12Q 2600/112: Disease subtyping, staging or classification
- C12Q 2600/118: Prognosis of disease development
- C12Q 2600/154: Methylation markers
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q 2600/158: Expression markers
- C12Q 2600/178: miRNA, siRNA or ncRNA
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Disclosed herein are methods for assaying a biological sample from a subject by analyzing components of microvesicle fractions in aid of risk, diagnosis, prognosis or monitoring of, or directing treatment of the subject for, a disease or other medical condition in the subject. Also disclosed are methods of treatment and identifying biomarkers using a microvesicle fraction of a subject. Kits, pharmaceutical compositions, and profiles related to the methods are also disclosed.

## Claims

### Claim 1

A method for assaying a biological sample from a subject in aid of diagnosis, prognosis or monitoring of a disease or other medical condition in the subject, comprising the steps of:
a. obtaining or using a microvesicle fraction from a biological sample from a subject;
b. extracting nucleic acid from the fraction; and
c. detecting the presence or absence of a biomarker in the extracted nucleic acid;
wherein the biomarker is a genetic aberration associated with diagnosis, prognosis, status or stage of a disease or other medical condition, and wherein the genetic aberration is in or corresponds to:
i. a c-myc gene;
ii. a transposable element;
iii. a retrotransposon element;
iv. a satellite correlated gene;
v. a repeated DNA element;
vi. non-coding RNA other than miRNA; or
vii. a fragment of any of the foregoing.