# US 2020/0399710 A1 · Method of identifying tumor specific macromolecular isoforms > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2020/0399710 A1 - Document ID: US20200399710A1 - Status: Published application - Kind: A1 - Application number: 16/969,168 - Filed: 2019-02-07 - Published: 2020-12-24 - Earliest claimed: 2018-02-12 - Assignees: Isommune, LLC - Applicants: Isommune, LLC - Inventors: Christian Barrett - Web page: https://patentgrep.com/patent/US20200399710A1 ## CPC - C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575) - C07K 16/30: from tumour cells - C12Q 2600/156: Polymorphic or mutational markers - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The present disclosure provides methods for the identification of tumor-specific isoforms of polymorphic gene expression products. Methods of using said isoforms in the diagnosis and/or treatment of cancers and neoplasias are also provided. ## Claims ### Claim 1 A method of making a synthetic polynucleotide, comprising: selecting a cellular compartment; identifying a first set of genetic sequences expressed in said cellular compartment; identifying a second set of genetic sequences expressed as multiple isoforms; generating a subset of said first and second sets of genetic sequences, said subset comprising sequences that are both (a) expressed in said cellular compartment and (b) capable of being expressed in multiple isoforms; and generating polynucleotide primers that are complementary to one or more sequences from said subset of genetic sequences, and may further comprise sequences providing for the use of universal sequencing primers and/or unique genetic identifiers. ### Claim 2 (depends on 1) The method of claim 1 further comprising amplifying one or more genetic sequences of said subset of genetic sequences from tumor and/or normal tissue. ### Claim 3 (depends on 1) The method of claim 1, further comprising identifying the protein products of said subset of genetic sequences in tumor and/or normal tissue. ### Claim 4 (depends on 1) The method of claim 1, further comprising isolating the protein products of said subset of genetic sequences from tumor tissue. ### Claim 5 (depends on 1) The method of claim 1, wherein said polynucleotide primers further comprise additional sequences outside of the region containing the sequence of said isoforms. ### Claim 6 (depends on 1) The method of claim 1, wherein said polynucleotide primers further comprise universal sequencing sites. ### Claim 7 (depends on 1) The method of claim 1, wherein said polynucleotide primers further comprise sequences for the identification of said primers or the amplification products thereof. ### Claim 8 A method of obtaining a tumor-specific biomarker, comprising: selecting a cellular compartment; identifying a first set of genetic sequences expressed in said cellular compartment; identifying a second set of genetic sequences expressed as multiple isoforms; and generating a subset of said first and second sets of genetic sequences, said subset comprising one or more genetic sequences known to be both (a) expressed in said cellular compartment and (b) capable of being expressed in multiple isoforms; and extracting, purifying, or expressing the product of one or more genetic sequences from said subset of genetic sequences. ### Claim 9 (depends on 8) The method of claim 8 further comprising amplifying one or more genetic sequences of said subset of genetic sequences from tumor and/or normal tissue. ### Claim 10 (depends on 8) The method of claim 8, further comprising identifying the protein products of said one or more genetic sequences of said subset of genetic sequences in tumor and/or normal tissue. ### Claim 11 (depends on 8) The method of claim 8, further comprising isolating the protein products of said one or more genetic sequences of said subset of genetic sequences from tumor tissue. ### Claim 12 (depends on 8) The method of claim 8, further comprising producing the protein products of said one or more genetic sequences of said subset of genetic sequences by heterologous expression. ### Claim 13 (depends on 8) The method of claim 8, wherein said polynucleotide primers further comprise universal sequencing sites. ### Claim 14 (depends on 8) The method of claim 8, wherein said polynucleotide primers further comprise sequences for the identification of said primers or the amplification products thereof. ### Claim 15 A method of generating antibodies against a tumor-specific biomarker, comprising: selecting a cellular compartment; identifying a first set of genetic sequences expressed in said cellular compartment; identifying a second set of genetic sequences expressed as multiple isoforms; and generating a subset of said first and second sets of genetic sequences, said subset comprising sequences that are both (a) expressed in said cellular compartment and (b) capable of being expressed in multiple isoforms; and extracting, purifying, or expressing the product of one or more genetic sequences from said subset of genetic sequences; and generating an antibody against said product. ### Claim 16 (depends on 15) The method of claim 15 further comprising amplifying said one or more genetic sequences from said subset of genetic sequences from tumor and/or normal tissue. ### Claim 17 (depends on 15) The method of claim 15, further comprising identifying the protein products of said one or more genetic sequences from said subset of genetic sequences in tumor and/or normal tissue. ### Claim 18 (depends on 15) The method of claim 15, further comprising isolating the protein products of said one or more genetic sequences from said subset of genetic sequences from tumor tissue. ### Claim 19 (depends on 15) The method of claim 15, further comprising producing the protein products of said genetic sequences by heterologous expression. ### Claim 20 (depends on 15) The method of claim 15, wherein said generating an antibody against said product further comprises immunizing an animal with said product. ### Claim at position 21 21-27. (canceled)