# US 2020/0399672 A1 · Immunochromatographic analysis device for chikungunya virus detection

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- Number: US 2020/0399672 A1
- Document ID: US20200399672A1
- Status: Published application
- Kind: A1
- Application number: 16/968,467
- Filed: 2019-02-08
- Published: 2020-12-24
- Earliest claimed: 2018-02-09
- Assignees: Osaka University
- Applicants: Osaka University; Tanaka Kikinzoku Kogyo K.K.
- Inventors: Tatsuo SHIODA; Emi NAKAYAMA; Keita SUZUKI; Hisahiko IWAMOTO
- Web page: https://patentgrep.com/patent/US20200399672A1

## CPC

- G01N 33/56983: {Viruses}
- C07K 16/10: RNA viruses
- C12M 1/34: Measuring or testing with condition measuring or sensing means, e.g. colony counters
- C12Q 1/04: Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)}
- G01N 33/543: with an insoluble carrier for immobilising immunochemicals
- G01N 33/569: for microorganisms, e.g. protozoa, bacteria, viruses
- G01N 33/58: involving labelled substances (G01N33/53 takes precedence)
- G01N 33/68: involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)}
- G01N 2333/181: Alphaviruses or Group A arboviruses, e.g. sindbis, VEE, EEE, WEE or semliki forest virus
- G01N 2469/10: Detection of antigens from microorganism in sample from host
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to an immunochromatographic analysis device for detecting chikungunya virus, including a sample loading part, a labeling substance retaining part, a chromatographic medium part having a detection part, and an absorption part, wherein the labeling substance retaining part contains a first antibody against the chikungunya virus, including a specific heavy chain variable region and a specific light chain variable region or an antigen-binding fragment thereof, and the detection part contains a second antibody against the chikungunya virus, including a specific heavy chain variable region and the light chain variable region or an antigen-binding fragment thereof, and a third antibody against the chikungunya virus, including a specific heavy chain variable region and the light chain variable region or an antigen-binding fragment thereof.

## Claims

### Claim 1

An immunochromatographic analysis device for detecting chikungunya virus, comprising a sample loading part, a labeling substance retaining part, a chromatographic medium part having a detection part, and an absorption part, wherein
the labeling substance retaining part contains the following antibody (A) or an antigen-binding fragment thereof, and
the detection part contains the following antibody (B) or an antigen-binding fragment thereof, and the following antibody (C) or an antigen-binding fragment thereof,
(A) an antibody against the chikungunya virus, including the following heavy chain variable region (1) and the following light chain variable region (2),
(1) a heavy chain variable region including a heavy chain complementarity determining region (CDRH) 1, CDRH2, and CDRH3,
CDRH1 being a polypeptide containing the following amino acid sequence (H1-A),
CDRH2 being a polypeptide containing the following amino acid sequence (H2-A), and
CDRH3 being a polypeptide containing the following amino acid sequence (H3-A),
(H1-A) the following amino acid sequence (H1-A1), (H1-A2), or (H1-A3),
(H1-A1) an amino acid sequence of SEQ ID NO: 1,
(H1-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 1,
(H1-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 1,
(H2-A) the following amino acid sequence (H2-A1), (H2-A2), or (H2-A3),
(H2-A1) an amino acid sequence of SEQ ID NO: 2,
(H2-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 2,
(H2-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 2,
(H3-A) the following amino acid sequence (H3-A1), (H3-A2), or (H3-A3),
(H3-A1) an amino acid sequence of SEQ ID NO: 3,
(H3-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 3,
(H3-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 3,
(2) a light chain variable region including a light chain complementarity determining region (CDRL) 1, CDRL2, and CDRL3,
CDRL1 being a polypeptide containing the following amino acid sequence (L1-A),
CDRL2 being a polypeptide containing the following amino acid sequence (L2-A), and
CDRL3 being a polypeptide containing the following amino acid sequence (L3-A),
(L1-A) the following amino acid sequence (L1-A1), (L1-A2), or (L1-A3),
(L1-A1) an amino acid sequence of SEQ ID NO: 4,
(L1-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 4,
(L1-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 4,
(L2-A) the following amino acid sequence (L2-A1), (L2-A2), or (L2-A3),
(L2-A1) an amino acid sequence of SEQ ID NO: 5,
(L2-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 5,
(L2-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 5,
(L3-A) the following amino acid sequence (L3-A1), (L3-A2), or (L3-A3),
(L3-A1) an amino acid sequence of SEQ ID NO: 6,
(L3-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 6,
(L3-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 6,
(B) an antibody against the chikungunya virus, including the following heavy chain variable region (3) and the light chain variable region (2),
(3) a heavy chain variable region including a heavy chain complementarity determining region (CDRH) 1, CDRH2, and CDRH3,
CDRH1 being a polypeptide containing the following amino acid sequence (H1-B),
CDRH2 being a polypeptide containing the following amino acid sequence (H2-B), and
CDRH3 being a polypeptide containing the following amino acid sequence (H3-B),
(H1-B) the following amino acid sequence (H1-B1), (H1-B2), or (H1-B3),
(H1-B1) an amino acid sequence of SEQ ID NO: 7,
(H1-B2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 7,
(H1-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 7,
(H2-B) the following amino acid sequence (H2-B1), (H2-B2), or (H2-B3),
(H2-B1) an amino acid sequence of SEQ ID NO: 8,
(H2-B2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 8,
(H2-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 8,
(H3-B) the following amino acid sequence (H3-B1), (H3-B2), or (H3-B3),
(H3-B1) an amino acid sequence of SEQ ID NO: 9,
(H3-B2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 9,
(H3-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 9,
(C) an antibody against the chikungunya virus, including the following heavy chain variable region (4) and the light chain variable region (2),
(4) a heavy chain variable region including CDRH1, CDRH2, and CDRH3,
CDRH1 being a polypeptide containing the following amino acid sequence (H1-C),
CDRH2 being a polypeptide containing the following amino acid sequence (H2-C), and
CDRH3 being a polypeptide containing the following amino acid sequence (H3-C),
(H1-C) the following amino acid sequence (H1-C1), (H1-C2), or (H1-C3),
(H1-C1) an amino acid sequence of SEQ ID NO: 10,
(H1-C2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 10,
(H1-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 10,
(H2-C) the following amino acid sequence (H2-C1), (H2-C2), or (H2-C3),
(H2-C1) an amino acid sequence of SEQ ID NO: 11,
(H2-C2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 11,
(H2-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 11,
(H3-C) the following amino acid sequence (H3-C1), (H3-C2), or (H3-C3),
(H3-C1) an amino acid sequence of SEQ ID NO: 12,
(H3-C2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 12,
(H3-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 12.

### Claim 2

An immunochromatographic analysis device for detecting chikungunya virus, comprising a sample loading part, a labeling substance retaining part, a chromatographic medium part having a detection part, and an absorption part, wherein
the labeling substance retaining part contains the following antibody (A) or an antigen-binding fragment thereof, and
the detection part contains the following antibody (B) or an antigen-binding fragment thereof, and the following antibody (C) or an antigen-binding fragment thereof,
(A) an antibody against the chikungunya virus, including the following heavy chain variable region (1) and the following light chain variable region (2),
(1) a heavy chain variable region including a polypeptide composed of the following amino acid sequence (H-A),
(H-A) the following amino acid sequence (H-A1), (H-A2), or (H-A3),
(H-A1) an amino acid sequence of SEQ ID NO: 13,
(H-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 13,
(H-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 13,
(2) a light chain variable region including a polypeptide composed of the following amino acid sequence (L-A),
(L-A) the following amino acid sequence (L-A1), (L-A2), or (L-A3),
(L-A1) an amino acid sequence of SEQ ID NO: 14,
(L-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 14,
(L-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 14,
(B) an antibody against the chikungunya virus, including the following heavy chain variable region (3) and the light chain variable region (2),
(3) a heavy chain variable region including a polypeptide composed of the following amino acid sequence (H-B),
(H-B) the following amino acid sequence (H-B1), (H-B2), or (H-B3),
(H-B1) an amino acid sequence of SEQ ID NO: 15,
(H-B2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 15,
(H-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 15,
(C) an antibody against the chikungunya virus, including the following heavy chain variable region (4) and the light chain variable region (2),
(4) a heavy chain variable region including a polypeptide composed of the following amino acid sequence (H-C),
(H-C) the following amino acid sequence (H-C1), (H-C2), or (H-C3),
(H-C1) an amino acid sequence of SEQ ID NO: 16,
(H-C2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 16,
(H-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 16.

### Claim 3

An immunochromatographic analysis device for detecting chikungunya virus, comprising a sample loading part, a labeling substance retaining part, a chromatographic medium part having a detection part, and an absorption part, wherein
the labeling substance retaining part contains the following antibody (A) or an antigen-binding fragment thereof, and
the detection part contains the following antibody (B) or an antigen-binding fragment thereof, and the following antibody (C) or an antigen-binding fragment thereof,
(A) an antibody against the chikungunya virus, including the following heavy chain (1) and the following light chain (2),
(1) a heavy chain including a polypeptide composed of the following amino acid sequence (HA),
(HA) the following amino acid sequence (HA1), (HA2), or (HA3),
(HA1) an amino acid sequence of SEQ ID NO: 17,
(HA2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 17,
(HA3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 17,
(2) a light chain including a polypeptide composed of the following amino acid sequence (LA),
(LA) the following amino acid sequence (LA1), (LA2), or (LA3),
(LA1) an amino acid sequence of SEQ ID NO: 18,
(LA2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 18,
(LA3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 18,
(B) an antibody against the chikungunya virus, including the following heavy chain (3) and the light chain (2),
(3) a heavy chain including a polypeptide composed of the following amino acid sequence (HB),
(HB) the following amino acid sequence (HB1), (HB2), or (HB3),
(HB1) an amino acid sequence of SEQ ID NO: 19,
(HB2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 19,
(HB3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 19,
(C) an antibody against the chikungunya virus, including the following heavy chain (4) and the light chain (2),
(4) a heavy chain including a polypeptide composed of the following amino acid sequence (HC),
(HC) the following amino acid sequence (HC1), (HC2), or (HC3),
(HC1) an amino acid sequence of SEQ ID NO: 20,
(HC2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 20,
(HC3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 20.

### Claim 4 (depends on 1)

The immunochromatographic analysis device according to claim 1, for detecting a chikungunya virus envelope glycoprotein of the chikungunya virus.

### Claim 5 (depends on 4)

The immunochromatographic analysis device according to claim 4, wherein the chikungunya virus envelope glycoprotein is chikungunya virus E1 protein.

### Claim 6 (depends on 1)

The immunochromatographic analysis device according to claim 1, wherein the antibody (A) or an antigen-binding fragment thereof, the antibody (B) or an antigen-binding fragment thereof, and the antibody (C) or an antigen-binding fragment thereof specifically bind to chikungunya virus having at least any one genotype of ECSA genotype, Asian genotype, and WA genotype.

### Claim 7 (depends on 1)

The immunochromatographic analysis device according to claim 1, wherein
the labeling substance retaining part further contains the following antibody (D) or an antigen-binding fragment thereof, and
the detection part further contains the following antibody (E) or an antigen-binding fragment thereof,
(D) an antibody against the chikungunya virus, including the following heavy chain variable region (1) and the following light chain variable region (2),
(1) a heavy chain variable region including a heavy chain complementarity determining region (CDRH) 1, CDRH2, and CDRH3,
CDRH1 being a polypeptide containing the following amino acid sequence (H1-D),
CDRH2 being a polypeptide containing the following amino acid sequence (H2-D), and
CDRH3 being a polypeptide containing the following amino acid sequence (H3-D),
(H1-D) the following amino acid sequence (H1-D1), (H1-D2), or (H1-D3),
(H1-D1) an amino acid sequence of SEQ ID NO: 21,
(H1-D2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 21,
(H1-D3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 21,
(H2-D) the following amino acid sequence (H2-D1), (H2-D2), or (H2-D3),
(H2-D1) an amino acid sequence of SEQ ID NO: 22,
(H2-D2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 22,
(H2-D3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 22,
(H3-D) the following amino acid sequence (H3-D1), (H3-D2), or (H3-D3),
(H3-D1) an amino acid sequence of SEQ ID NO: 23,
(H3-D2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 23,
(H3-D3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 23,
(2) a light chain variable region including a light chain complementarity determining region (CDRL) 1, CDRL2, and CDRL3,
CDRL1 being a polypeptide containing the following amino acid sequence (L1-A),
CDRL2 being a polypeptide containing the following amino acid sequence (L2-A), and
CDRL3 being a polypeptide containing the following amino acid sequence (L3-A),
(L1-A) the following amino acid sequence (L1-A1), (L1-A2), or (L1-A3),
(L1-A1) an amino acid sequence of SEQ ID NO: 4,
(L1-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 4,
(L1-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 4,
(L2-A) the following amino acid sequence (L2-A1), (L2-A2), or (L2-A3),
(L2-A1) an amino acid sequence of SEQ ID NO: 5,
(L2-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 5,
(L2-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 5,
(L3-A) the following amino acid sequence (L3-A1), (L3-A2), or (L3-A3),
(L3-A1) an amino acid sequence of SEQ ID NO: 6,
(L3-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 6,
(L3-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 6,
(E) an antibody against the chikungunya virus, including the following heavy chain variable region (3) and the light chain variable region (2),
(3) a heavy chain variable region including a heavy chain complementarity determining region (CDRH) 1, CDRH2, and CDRH3,
CDRH1 being a polypeptide containing the following amino acid sequence (H1-E),
CDRH2 being a polypeptide containing the following amino acid sequence (H2-E), and
CDRH3 being a polypeptide containing the following amino acid sequence (H3-E),
(H1-E) the following amino acid sequence (H1-E1), (H1-E2), or (H1-E3),
(H1-E1) an amino acid sequence of SEQ ID NO: 24,
(H1-E2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 24,
(H1-E3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 24,
(H2-E) the following amino acid sequence (H2-E1), (H2-E2), or (H2-E3),
(H2-E1) an amino acid sequence of SEQ ID NO: 25,
(H2-E2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 25,
(H2-E3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 25,
(H3-E) the following amino acid sequence (H3-E1), (H3-E2), or (H3-E3),
(H3-E1) an amino acid sequence of SEQ ID NO: 26,
(H3-E2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 26,
(H3-E3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 26.

### Claim 8 (depends on 1)

The immunochromatographic analysis device according to claim 1, wherein
the labeling substance retaining part further contains the following antibody (D) or an antigen-binding fragment thereof, and
the detection part further contains the following antibody (E) or an antigen-binding fragment thereof,
(D) an antibody against the chikungunya virus, including the following heavy chain variable region (1) and the following light chain variable region (2),
(1) a heavy chain variable region including a polypeptide composed of the following amino acid sequence (H-D),
(H-D) the following amino acid sequence (H-D1), (H-D2), or (H-D3),
(H-D1) an amino acid sequence of SEQ ID NO: 27,
(H-D2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 27,
(H-D3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 27,
(2) a light chain variable region including a polypeptide composed of the following amino acid sequence (L-A),
(L-A) the following amino acid sequence (L-A1), (L-A2), or (L-A3),
(L-A1) an amino acid sequence of SEQ ID NO: 14,
(L-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 14,
(L-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 14,
(E) an antibody against the chikungunya virus, including the following heavy chain variable region (3) and the light chain variable region (2),
(3) a heavy chain variable region including a polypeptide composed of the following amino acid sequence (H-E),
(H-E) the following amino acid sequence (H-E1), (H-E2), or (H-E3),
(H-E1) an amino acid sequence of SEQ ID NO: 28,
(H-E2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 28,
(H-E3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 28.

### Claim 9 (depends on 1)

The immunochromatographic analysis device according to claim 1, wherein
the labeling substance retaining part further contains the following antibody (D) or an antigen-binding fragment thereof, and
the detection part further contains the following antibody (E) or an antigen-binding fragment thereof,
(D) an antibody against the chikungunya virus, including the following heavy chain (1) and the following light chain (2),
(1) a heavy chain including a polypeptide composed of the following amino acid sequence (HD),
(HD) the following amino acid sequence (HD1), (HD2), or (HD3),
(HD1) an amino acid sequence of SEQ ID NO: 29,
(HD2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 29,
(HD3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 29,
(2) a light chain including a polypeptide composed of the following amino acid sequence (LA),
(LA) the following amino acid sequence (LA1), (LA2), or (LA3),
(LA1) an amino acid sequence of SEQ ID NO: 18,
(LA2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 18,
(LA3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 18,
(E) an antibody against the chikungunya virus, including the following heavy chain (3) and the light chain (2),
(3) a heavy chain including a polypeptide composed of the following amino acid sequence (HE),
(HE) the following amino acid sequence (HE1), (HE2), or (HE3),
(HE1) an amino acid sequence of SEQ ID NO: 30,
(HE2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO: 30,
(HE3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 30.

### Claim 10 (depends on 7)

The immunochromatographic analysis device according to claim 7, wherein the antibody (D) or an antigen-binding fragment thereof, and the antibody (E) or an antigen-binding fragment thereof specifically bind to chikungunya virus having at least any one genotype of ECSA genotype, Asian genotype, and WA genotype.

### Claim 11 (depends on 7)

The immunochromatographic analysis device according to claim 7, wherein a content ratio (mass) of the antibody (A) or an antigen-binding fragment thereof to the antibody (D) or an antigen-binding fragment thereof in the labeling substance retaining part is 1:2 to 2:1.

### Claim 12 (depends on 1)

The immunochromatographic analysis device according to claim 1, wherein a sample to be loaded in the sample loading part is any one of the whole blood, serum, plasma, semen, and spinal fluid of a chikungunya virus infected individual.

### Claim 13 (depends on 1)

An immunochromatographic analysis kit, comprising the immunochromatographic analysis device according to claim 1, and a specimen diluent for diluting and developing a specimen.

### Claim 14 (depends on 13)

An immunochromatographic analysis method for detecting chikungunya virus contained in a specimen using the immunochromatographic analysis kit according to claim 13, comprising the following steps (1) to (4):
(1) a step of loading a specimen-containing liquid obtained by diluting a specimen with a specimen diluent in the sample loading part;
(2) a step of allowing the antibody (A) or an antigen-binding fragment thereof held by the labeling substance retaining part to recognize the chikungunya virus;
(3) a step of developing the specimen and the antibody (A) or an antigen-binding fragment thereof in the chromatographic medium part as a mobile phase; and
(4) a step of detecting the chikungunya virus in the developed mobile phase with the antibody (B) or an antigen-binding fragment thereof and the antibody (C) or an antigen-binding fragment thereof contained in the detection part.

### Claim 15 (depends on 2)

The immunochromatographic analysis device according to claim 2, for detecting a chikungunya virus envelope glycoprotein of the chikungunya virus.

### Claim 16 (depends on 3)

The immunochromatographic analysis device according to claim 3, for detecting a chikungunya virus envelope glycoprotein of the chikungunya virus.

### Claim 17 (depends on 15)

The immunochromatographic analysis device according to claim 15, wherein the chikungunya virus envelope glycoprotein is chikungunya virus E1 protein.

### Claim 18 (depends on 16)

The immunochromatographic analysis device according to claim 16, wherein the chikungunya virus envelope glycoprotein is chikungunya virus E1 protein.

### Claim 19 (depends on 2)

The immunochromatographic analysis device according to claim 2, wherein the antibody (A) or an antigen-binding fragment thereof, the antibody (B) or an antigen-binding fragment thereof, and the antibody (C) or an antigen-binding fragment thereof specifically bind to chikungunya virus having at least any one genotype of ECSA genotype, Asian genotype, and WA genotype.

### Claim 20 (depends on 3)

The immunochromatographic analysis device according to claim 3, wherein the antibody (A) or an antigen-binding fragment thereof, the antibody (B) or an antigen-binding fragment thereof, and the antibody (C) or an antigen-binding fragment thereof specifically bind to chikungunya virus having at least any one genotype of ECSA genotype, Asian genotype, and WA genotype.