# US 2020/0399658 A1 · Electroporation cuvettes for automation

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- Number: US 2020/0399658 A1
- Document ID: US20200399658A1
- Status: Published application
- Kind: A1
- Application number: 17/013,494
- Filed: 2020-09-04
- Published: 2020-12-24
- Earliest claimed: 2017-08-28
- Assignees: Inscripta, Inc.
- Applicants: Inscripta, Inc.
- Inventors: Jorge Bernate; Phil Belgrader; Don Masquelier; Vlorent Morina
- Web page: https://patentgrep.com/patent/US20200399658A1

## CPC

- C12N 15/87: Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12M 25/02: {Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00)}
- C12M 35/02: {Electrical or electromagnetic means, e.g. for electroporation or for cell fusion}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

The present disclosure relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment.

## Claims

### Claim 1

An automated cell processing system comprising:
a growth module;
an electroporation module comprising an automated liquid handling device and a multi-unit electroporation module, wherein each electroporation module comprises
a housing configured to engage with a pipette from the automated liquid handling device;
an electroporation cuvette comprising an electroporation chamber defined by walls and wherein the electroporation cuvette is configured to receive a liquid from the automated liquid handling device; and
a sipper in fluid communication with the electroporation chamber, wherein the sipper is configured for intake and output of a sample comprising cells to be electroporated in the electroporation cuvette; and
a processor.

### Claim 2 (depends on 1)

The automated cell processing system of claim 1, wherein the pipette is an automatic air displacement pipette.

### Claim 3 (depends on 1)

The automated cell processing system of claim 1, wherein the pipette is a multi-channel pipette.

### Claim 4 (depends on 1)

The automated cell processing system of claim 1, wherein the sample comprises bacterial cells.

### Claim 5 (depends on 1)

The automated cell processing system of claim 1, wherein the sample comprises mammalian cells.

### Claim 6 (depends on 1)

The automated cell processing system of claim 1, wherein the liquid comprises one or more materials to be electroporated into the cells selected from nucleic acids, peptides, proteins, hormones, cytokines, chemokines, drugs, or drug precursors.

### Claim 7 (depends on 6)

The automated cell processing system of claim 6, wherein the liquid comprises nucleic acids.

### Claim 8 (depends on 1)

The automated processing system of claim 1, wherein the liquid comprises at least one reagent of an RNA-directed nuclease editing system.

### Claim 9 (depends on 1)

The automated cell processing system of claim 1, wherein the processor is configured to read an optical density (OD) of the cells in the growth module.

### Claim 10 (depends on 9)

The automated cell processing system of claim 9, wherein the processor is configured to instruct the growth module that the cells have reached a target OD.

### Claim 11 (depends on 1)

The automated cell processing system of claim 1, wherein the processor is configured to provide input for the pipette to move the cells into the electroporation module after cell growth in the growth module.

### Claim 12 (depends on 1)

The automated cell processing system of claim 1, wherein the processor is configured to provide input for each electroporation module for dispensing of reagents via the automated liquid handling device.

### Claim 13 (depends on 1)

The automated cell processing system of claim 1, wherein the processor is configured to provide input for each electroporation module for the time, voltage or waveform for electroporation.

### Claim 14 (depends on 1)

The automated cell processing system of claim 1, further comprising a storage module.

### Claim 15 (depends on 14)

The automated cell processing system of claim 14, wherein the storage module stores the cells at 4° C. or lower temperatures.

### Claim 16 (depends on 1)

The automated cell processing system of claim 1, further comprising a filtration module.

### Claim 17 (depends on 1)

The automated cell processing system of claim 1, further comprising a nucleic acid assembly module.

### Claim 18 (depends on 17)

The automated cell processing system of claim 17, further comprising a nucleic acid purification module.

### Claim 19 (depends on 1)

The automated cell processing system of claim 1, further comprising a separation and concentration module.

### Claim 20 (depends on 19)

The automated cell processing system of claim 19 further comprising a recovery module.