# US 2020/0399653 A1 · Insecticidal proteins and methods for their use

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2020/0399653 A1
- Document ID: US20200399653A1
- Status: Published application
- Kind: A1
- Application number: 16/918,015
- Filed: 2020-07-01
- Published: 2020-12-24
- Earliest claimed: 2014-10-16
- Assignees: Pioneer Hi-Bred International, Inc.; E. I. Du Pont de Nemours and Company
- Applicants: Pioneer Hi-Bred International, Inc.; E. I. Du Pont De Nemours and Company
- Inventors: JENNIFER KARA BARRY; DEBORAH CLARK; JAMES J ENGLISH; AZALEA S ONG; ERIC SCHEPERS; JULIE QI; JANET RICE
- Web page: https://patentgrep.com/patent/US20200399653A1

## CPC

- C07K 14/415: from plants
- C12N 15/8286: {for insect resistance}
- A01N 37/46: N-acyl derivatives
- A01N 65/04: Pteridophyta [fern allies]; Filicophyta [ferns]
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

## Claims

### Claim 1

A recombinant PtIP-96 polypeptide having at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 6, SEQ ID NO: 10, SEQ ID NO: 42 or SEQ ID NO: 54.

### Claim 2 (depends on 1)

The recombinant PtIP-96 polypeptide of claim 1, wherein the PtIP-96 polypeptide has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 6, SEQ ID NO: 10, SEQ ID NO: 42 or SEQ ID NO: 54.

### Claim 3 (depends on 1)

The recombinant PtIP-96 polypeptide of claim 1, wherein the PtIP-96 polypeptide has insecticidal activity against Corn Earworm (Helicoverpa zea).

### Claim 4 (depends on 1)

A recombinant polynucleotide encoding the PtIP-96 polypeptide of claim 1.

### Claim 5 (depends on 4)

The recombinant polynucleotide of claim 4, wherein the polynucleotide is a non-genomic polynucleotide.

### Claim 6 (depends on 5)

The recombinant polynucleotide of claim 5, wherein the polynucleotide is a cDNA.

### Claim 7 (depends on 5)

The recombinant polynucleotide of claim 5, wherein the polynucleotide is synthetic polynucleotide.

### Claim 8 (depends on 7)

The recombinant polynucleotide of claim 7, wherein the polynucleotide has codons optimized for expression in an agriculturally important crop.

### Claim 9 (depends on 4)

A transgenic plant or plant cell comprising the polynucleotide of claim 4.

### Claim 10 (depends on 4)

A DNA construct comprising the polynucleotide of claim 4 operably linked to a heterologous regulatory element.

### Claim 11 (depends on 10)

A transgenic plant or plant cell comprising the DNA construct of claim 10.

### Claim 12 (depends on 1)

A composition comprising the PtIP-96 polypeptide of claim 1.

### Claim 13 (depends on 1)

A fusion protein comprising the PtIP-96 polypeptide of claim 1.

### Claim 14 (depends on 1)

A method for controlling an insect pest population, comprising contacting the insect pest population with the PtIP-96 polypeptide of claim 1.

### Claim 15 (depends on 1)

A method of inhibiting growth or killing an insect pest, comprising contacting the insect pest with a composition comprising the PtIP-96 polypeptide of claim 1.

### Claim 16 (depends on 1)

A method of controlling Lepidoptera and/or Coleoptera insect infestation in a transgenic plant and providing insect resistance management, comprising expressing in the plant the PtIP-96 polypeptide of claim 1.

### Claim 17 (depends on 11)

A method for controlling an insect pest population, comprising contacting the insect pest population with the transgenic plant or plant cell of claim 11.

### Claim 18 (depends on 10)

A method of inhibiting growth or killing an insect pest, comprising transforming a plant with the DNA construct of claim 10.

### Claim 19 (depends on 18)

The method of claim 18, further comprising contacting the insect pest with the transgenic plant or plant cell.

### Claim 20 (depends on 18)

The method of claim 18, wherein the insect pest is Corn Earworm (Helicoverpa zea).

### Claim 21 (depends on 17, 18, 19, 20)

The method of claim 17, 18, 19 or 20, wherein the insect pest or insect pest population is resistant to at least one Bt toxin.

### Claim 22

(canceled)