# US 2020/0399652 A1 · Tbrfv resistant tomato plant

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- Number: US 2020/0399652 A1
- Document ID: US20200399652A1
- Status: Published application
- Kind: A1
- Application number: 15/930,651
- Filed: 2020-05-13
- Published: 2020-12-24
- Earliest claimed: 2017-12-08
- Assignees: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Applicants: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Inventors: Roel Hamelink; Jonathan Kalisvaart; Hamed Rashidi
- Web page: https://patentgrep.com/patent/US20200399652A1

## CPC

- A01H 1/126: {for virus resistance}
- A01H 1/045: {using molecular markers}
- A01H 5/08: Fruits
- A01H 6/825: {Solanum lycopersicum [tomato]}
- C12N 15/8283: {for virus resistance}
- C12Q 1/6895: for plants, fungi or algae
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The invention relates to a Solanum lycopersicum plant that is resistant to TBRFV, which plant comprises a QTL on chromosome 11 between SEQ ID NO: 1 and 53. The presence of the QTL on chromosome 11 is identified by use of at least one of the markers selected from the group comprising SEQ ID NOS: 4 to 52. The QTL is as comprised in the genome of a Solanum lycopersicum plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 42882, NCIMB 42885, NCIMB 42887, or NCIMB 42890.

## Claims

### Claim 1

A Solanum lycopersicum plant that is resistant to TBRFV, which plant comprises a QTL on chromosome 11, wherein the QTL on chromosome 11 is located between SEQ ID NO: 1 and SEQ ID NO: 53.

### Claim 2 (depends on 1)

A Solanum lycopersicum plant as claimed in claim 1, wherein the presence of the QTL on chromosome 11 is identified by use of at least one of the markers selected from the group comprising SEQ ID NOS: 4 to 52.

### Claim 3 (depends on 1)

A Solanum lycopersicum plant as claimed in claim 1, wherein the QTL is flanked by SEQ ID NO: 4 and SEQ ID NO: 52 in the genome of a Solanum lycopersicum plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 42882, NCIMB 42885, NCIMB 42887, or NCIMB 42890.

### Claim 4 (depends on 1, 2, 3)

A cell of a TBRFV resistant Solanum lycopersicum plant as claimed in claim 1, which cell comprises the QTL as defined in any of the claims 1-3 on chromosome 11 in its genome.

### Claim 5 (depends on 1)

A Solanum lycopersicum seed, wherein a plant grown from the seed is resistant to TBRFV due to the presence in its genome of the QTL on chromosome 11 as defined claim 1.

### Claim 6 (depends on 1)

A propagation material suitable for producing a Solanum lycopersicum plant as claimed in any claim 1, wherein the propagation material is suitable for sexual reproduction, vegetative reproduction or for tissue culture of regenerable cells, wherein the plant produced from the propagation material comprises the QTL that leads to TBRFV resistance on chromosome 11, as defined in claim 1.

### Claim 7 (depends on 6)

The propagation material of claim 6, wherein the propagation material suitable for sexual reproduction is selected from the group comprising a microspore, pollen, an ovary, an ovule, an embryo sac, and an egg cell.

### Claim 8 (depends on 6)

The propagation material of claim 6, wherein the propagation material suitable for wherein the propagation material is suitable for vegetative reproduction is selected from the group comprising a cutting, a root, a stem, a cell, and a protoplast.

### Claim 9 (depends on 6)

The propagation material of claim 6, wherein the propagation material suitable for wherein the propagation material is suitable for tissue culture of regenerable cells and is selected from the group comprising a leaf, pollen, an embryo, a cotyledon, a hypocotyl, a meristematic cell, a root, a root tip, an anther, a flower, a seed, and a stem.

### Claim 10

A marker for the identification of TBRFV resistance in a Solanum lycopersicum plant, which marker is selected from the group comprising SEQ ID NOS: 4 to 52.

### Claim 11

A marker for the identification of a QTL on chromosome 11 that leads to TBRFV resistance in a Solanum lycopersicum plant, which marker is selected from the group comprising SEQ ID NOS: 4 to 52.

### Claim 12 (depends on 10)

A method for identifying TBRFV resistance in a Solanum lycopersicum plant comprising identifying the marker of claim 10 in the plant.

### Claim 13 (depends on 11)

A method for identifying TBRFV resistance in a Solanum lycopersicum plant comprising identifying the marker of claim 11 in the plant.

### Claim 14 (depends on 1)

A method for producing a TBRFV resistant Solanum lycopersicum plant comprising introducing a QTL as defined in claim 1 on chromosome 11, in a S. lycopersicum plant.

### Claim 15 (depends on 1)

A method for selecting a TBRFV resistant Solanum lycopersicum plant, comprising identifying the presence of a QTL as defined in claim 1 on chromosome 11, and selecting a plant that comprises said QTL as a TBRFV resistant plant.

### Claim 16 (depends on 15)

The method as claimed in claim 15, wherein identifying the presence of the QTL on chromosome 11 is done using a marker selected from the group comprising SEQ ID NOS: 4 to 52.

### Claim 17 (depends on 1)

A method for the production of a Solanum lycopersicum plant which is resistant to TBRFV, said method comprising:
a) crossing the plant as claimed in claim 1 comprising the QTL on chromosome 11 with another plant;
b) optionally performing one or more rounds of selfing and/or crossing of the plant resulting from the cross in step a) to obtain a further generation population;
c) selecting from the plant resulting from the cross in step a), or from the further generation population of step b), a plant that comprises the QTL on chromosome 11, as defined in claim 1, which plant is resistant against TBRFV.

### Claim 18 (depends on 17)

The method as claimed in claim 17, wherein selection of a plant comprising the QTL on chromosome 11 is done by using a molecular marker genetically linked to the QTL, which marker is selected from the group comprising SEQ ID NOS: 4 to 52.

### Claim 19 (depends on 17)

The method as claimed in claim 17, wherein the plant which is resistant to TBRFV is phenotypically selected, in particular by using a bio-assay for TBRFV resistance.

### Claim 20 (depends on 17)

The method as claimed in claim 17, wherein the plant from step a) is a plant grown from seed deposited under NCIMB accession number NCIMB 42882, NCIMB 42885, NCIMB 42887, or NCIMB 42890, or a progeny plant thereof.

### Claim 21 (depends on 1)

A method for the production of hybrid seed comprising crossing a first parent plant with a second parent plant and harvesting the resultant hybrid seed, wherein the first parent plant and/or the second parent plant is a plant of the invention that is resistant to TBRFV comprising the QTL as defined in claim 1 on chromosome 11, wherein the presence of the QTL leads to resistance to TBRFV in the hybrid plant that is grown from the seed.