# US 2020/0399651 A1 · Plants having increased tolerance to herbicides > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2020/0399651 A1 - Document ID: US20200399651A1 - Status: Published application - Kind: A1 - Application number: 16/962,562 - Filed: 2019-01-15 - Published: 2020-12-24 - Earliest claimed: 2018-01-17 - Assignees: BASF SE - Applicants: BASF SE - Inventors: Doreen Schachtschabel; Eva Hollenbach; Mihiret Tekeste Sisay; Thomas Zierke; Danny Geerdink - Web page: https://patentgrep.com/patent/US20200399651A1 ## CPC - C12N 15/8274: {for herbicide resistance} - A01H 1/00: Processes for modifying genotypes {; Plants characterised by associated natural traits} (A01H4/00 takes precedence) - C12N 9/78: acting on carbon to nitrogen bonds other than peptide bonds (3.5) - C12N 15/00: Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00) - C12Y 305/04: in cyclic amidines (3.5.4) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - C12Y: ENZYMES ## Abstract Provided is a plant or plant part comprising a polynucleotide encoding a mutant TriA polypeptide, the expression of the polynucleotide confers to the plant or plant part tolerance to herbicides ## Claims ### Claim 1 A plant or plant part comprising a polynucleotide encoding a mutated TriA polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to herbicides. ### Claim 2 (depends on 1) The plant or plant part of claim 1, wherein the herbicide comprises a compound which inhibits cellulose biosynthesis ### Claim 3 (depends on 1) The plant or plant part of claim 1, wherein the polynucleotide encoding the mutated TriA polypeptide comprises the nucleic acid sequence set forth in SEQ ID NO: 1, or a homologue, variant or derivative thereof. ### Claim 4 (depends on 1) The plant or plant part of claim 1, wherein the mutated TriA polypeptide is a functional variant having, over the full-length of the variant, at least about 60%, illustratively, at least about 80%, 90%, 95%, 98%, 99% or more amino acid sequence identity to SEQ ID NO: 2. ### Claim 5 (depends on 1) The plant or plant part of claim 1, wherein the mutated TriA refers to a TriA polypeptide comprising the sequence of SEQ ID NO: 2, an orthologue, paralogue, or homologue thereof, wherein the amino acid sequence differs from the wildtype amino acid sequence at one or more positions corresponding to positions 92, 93, 155, 157 of SEQ ID NO: 2. ### Claim 6 A seed capable of germination into a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides. ### Claim 7 A plant cell of or capable of regenerating a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides, wherein the plant cell comprises the polynucleotide operably linked to a promoter. ### Claim 8 A plant cell comprising a polynucleotide operably linked to a promoter operable in a cell, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides. ### Claim 9 A plant product prepared from a plant or plant part comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides. ### Claim 10 A progeny or descendant plant derived from a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, wherein the progeny or descendant plant comprises in at least some of its cells the polynucleotide operably linked to the promoter, the expression of the mutated TriA polypeptide conferring to the progeny or descendant plant tolerance to the herbicides. ### Claim 11 A method for controlling weeds at a locus for growth of a plant, the method comprising: (a) applying an herbicide composition comprising herbicides to the locus; and (b) planting a seed at the locus, wherein the seed is capable of producing a plant that comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides. ### Claim 12 (depends on 11) The method of claim 11, wherein herbicide composition is applied to the weeds and to the plant produced by the seed. ### Claim 13 (depends on 11) The method according to claim 11, wherein the herbicide comprises a compound which inhibits cellulose biosynthesis. ### Claim 14 A method of producing a plant having tolerance to herbicide, the method comprising regenerating a plant from a plant cell transformed with a recombinant polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides. ### Claim 15 A method of producing a progeny plant having tolerance to herbicide, the method comprising: crossing a first herbicide-tolerant plant with a second plant to produce a herbicide-tolerant progeny plant, wherein the first plant and the progeny plant comprise in at least some of their cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides. ### Claim 16 A plant or plant part comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait. ### Claim 17 An isolated and/or recombinantly produced and/or synthetic nucleic acid molecule comprising a nucleic acid molecule encoding a mutated TriA polypeptide selected from the group consisting of: (a) a nucleic acid molecule encoding a mutated TriA polypeptide comprising the sequence of SEQ ID NO: 2, or a variant, paralogue, orthologue or homolog thereof; (b) a nucleic acid molecule comprising the sequence of SEQ ID NO: 1, or a variant, paralogue, orthologue or homolog thereof; (c) a nucleic acid molecule, which, as a result of the degeneracy of the genetic code, can be derived from a TriA polypeptide sequence SEQ ID NO: 2, or a variant, paralogue, orthologue or homolog thereof, and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof; (d) a nucleic acid molecule having 30% or more identity, preferably 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99,5%, or more with the nucleic acid molecule sequence of a polynucleotide comprising the nucleic acid molecule of SEQ ID NO: 1, or a variant, paralogue, orthologue or homolog thereof, and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof; (e) a nucleic acid molecule encoding a mutated TriA polypeptide having 30% or more identity, preferably at least 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99,5% or more, with the amino acid sequence of the TriA polypeptide sequence SEQ ID NO: 2, and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof; nucleic acid molecule which hybridizes with a nucleic acid molecule of (a), (b), (c), (d) or (e) under stringent hybridization conditions and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof; wherein the amino acid sequence of the mutated TriA polypeptide differs from the wildtype amino acid sequence of a TriA polypeptide at one or more positions corresponding to the following positions of SEQ ID NO:2: 92, 93, 155, 157. ### Claim 18 (depends on 17) An expression cassette comprising the nucleic acid molecule as defined in claim 17, and a promoter operable in plant cells. ### Claim 19 (depends on 18) The expression cassette of claim 18, wherein the promoter is a root-specific promoter. ### Claim 20 (depends on 17) A vector comprising the nucleic acid molecule as defined in claim 17. ### Claim 21 (depends on 17) An isolated, recombinant and/or chemically synthesized mutated TriA polypeptide encoded by the nucleic acid molecule as claimed in claim 17 or a polypeptide having at least 80%, 90%, 95%, 98%, 99% identity to the sequence of SEQ ID NO: 2, or thereof, wherein the amino acid sequence of the mutated TriA polypeptide differs from the wildtype amino acid sequence of a TriA polypeptide at one or more positions corresponding to the following positions of SEQ ID NO:2: 92, 93, 155, 157. ### Claim 22 (depends on 1) A method for producing a plant product from the plant of claim 1, the method comprising processing the plant or a plant part thereof to obtain the plant product. ### Claim 23 (depends on 22) The method of claim 22, wherein the plant product is fodder, seed meal, oil, or seed-treatment-coated seeds. ### Claim 24 A plant product obtained from a plant or plant part thereof, wherein the plant or plant part comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait. ### Claim 25 (depends on 24) The plant product of claim 24, wherein the product is fodder, seed meal, oil, or seed-treatment-coated seed.