Methods for generating crop yield traits
AssigneeEnobraq
InventorsCedric BOISART, Nicolas CHABOT
Life of this patent
Estimating the term…
Abstract
The present technology relates to a method for generating crop yield traits. The method comprises identifying a multi-genic system associated with one or more gene of interest, wherein the one or more gene of interest is operatively linked to yield of crop. Identifying at least one combination of genes within the multi-genic system that yields an active multi-protein system of interest and generating mutational diversity on the at least one combination of genes to generate a library. The method further comprises screening the library to identify mutations yielding an improved activity of multi-protein system of interest in an expression system and introducing the screened mutations into the crop.
Claims19
A method for generating yield traits to improve crop yield, the method comprising:
- a) identifying a multi-genic system associated with one or more gene of interest, wherein the one or more gene of interest is operatively linked to yield of crop;
- b) identifying at least one combination of genes within the multi-genic system that yields an active multi-protein system of interest;
- c) generating mutational diversity on the at least one combination of genes to generate a library;
- d) screening the library to identify mutations yielding an improved activity of multi-protein system of interest in an expression system;
- and
- e) introducing the screened mutations of step d) into the crop.
A method for generating yield traits to improve crop yield, the method comprising:
- a) identifying a multi-genic system associated with one or more gene of interest, wherein the one or more gene of interest is operatively linked to crop yield;
- b) expressing in an expression system a plurality of combinations of genes from crop that are homologous to the multi-genic system to obtain a first library;
- c) screening the first library for at least one combination of genes yielding the active multi-genic system of interest in the expression system;
- d) integrating the at least one combination of genes in the expression system to carry out an adaptive laboratory evolution and to generate an evolved population of the expression system;
- e) screening the evolved population to identify mutations resulting in an improved activity of the multi-genic system of interest;
- and
- f) introducing the screened mutations of step e) into the crop.
Dependencies are inferred from the claim text.