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US 2020/0399586 A1Published application
Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
AssigneeInscripta, Inc.
InventorsRichard Fox, Daniel Held, Eric Abbate, Michael Clay, Katherine Krouse, Nandini Krishnamurthy, Krishna Yerramsetty
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Abstract
The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.
Claims20
An E. coli cell with a dapA protein having an amino acid sequence of SEQ ID No. 1, a dapA gene promoter sequence of nucleic acid SEQ ID No. 2 and an additional protein selected from the following proteins:
- a lysC protein having an amino acid sequence of SEQ ID No. 3, a garD protein coding sequence having a nucleic acid sequence of SEQ ID No. 4, a yicL protein coding sequence having a nucleic acid sequence of SEQ ID No. 5, a lysP protein having an amino acid sequence of SEQ ID No. 6, a mgSA protein coding sequence having a nucleic acid sequence of SEQ ID No. 7, or a pckE protein having an amino acid sequence of SEQ ID No. 8.
An E. coli cell with a dapA gene having a promoter sequence of nucleic acid SEQ ID No. 2 and one of the following proteins:
- an amyA protein coding sequence having a nucleic acid sequence of SEQ ID No. 9, an amyA protein having an amino acid sequence of SEQ ID No. 10, a cysN protein having an amino acid sequence of SEQ ID No. 11, a dosP protein coding sequence having a nucleic acid sequence of SEQ ID No. 12, a emrE protein coding sequence having a nucleic acid sequence SEQ ID No. 13, a focB protein coding sequence having a nucleic acid sequence SEQ ID No. 14, a glnD protein coding sequence having a nucleic acid sequence SEQ ID No. 15, a glnE protein having an amino acid sequence of SEQ ID No. 16, a hicB protein coding sequence having a nucleic acid sequence of SEQ ID No. 17, a maeB protein coding sequence having a nucleic acid sequence of SEQ ID No. 18, a marA protein having an amino acid sequence of SEQ ID No. 19, or a metL protein having an amino acid sequence of SEQ ID No. 20.
Dependencies are inferred from the claim text.