# US 2020/0399562 A1 · Method of treating a fabric > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2020/0399562 A1 - Document ID: US20200399562A1 - Status: Published application - Kind: A1 - Application number: 17/005,499 - Filed: 2020-08-28 - Published: 2020-12-24 - Earliest claimed: 2015-04-29 - Assignees: The Procter & Gamble Company - Applicants: The Procter & Gamble Company - Inventors: Neil Joseph Lant; Rebecca Louise Wood; Jeremie Robert Marcel Gummel - Web page: https://patentgrep.com/patent/US20200399562A1 ## CPC - C11D 1/62: Quaternary ammonium compounds - C11D 3/0015: {liquid} - C11D 3/373: {containing silicones} - C11D 3/3749: {Polyolefins; Halogenated polyolefins; Natural or synthetic rubber; Polyarylolefins or halogenated polyarylolefins} - C11D 3/38636: {containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase} - D06M 13/46: Compounds containing quaternary nitrogen atoms (hydrazinium compounds D06M13/338; betaines, sulfo-betaines D06M13/342) - D06M 15/227: of hydrocarbons, or reaction products thereof, e.g. afterhalogenated or sulfochlorinated - D06M 15/6436: {containing amino groups} - D06M 16/003: {with enzymes or microorganisms} - C11D 2111/12: Soft surfaces, e.g. textile - C12Y 301/30: Endoribonucleases active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters (3.1.30) - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - D06M: TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS - C12Y: ENZYMES ## Abstract A method of treating a fabric, comprising contacting the fabric with an aqueous liquor comprising a fabric care component selected from the group consisting of cationic softening-compounds, silicone softening-compounds, paraffins, dispersible polyolefins, waxes and mixtures thereof; and contacting the fabric with an aqueous liquor comprising a nuclease enzyme, preferably a deoxyribonuclease or ribonuclease enzyme. The aqueous liquor may be provided by adding a cleaning or treatment composition to water. Preferably the composition comprises a surfactant, the wash liquor comprising from 0.05 to 4 g/l of a surfactant. ## Claims ### Claim 1 A method of treating a fabric, comprising (i) contacting the fabric with an aqueous liquor comprising a fabric care component selected from the group consisting of cationic softening-compounds, silicone softening-compounds, paraffins, waxes, dispersible polyolefins and mixtures thereof; and (ii) contacting the fabric with an aqueous liquor comprising a nuclease enzyme. ### Claim 2 (depends on 1) A method according to claim 1 wherein the nuclease enzyme comprises a deoxyribonuclease or ribonuclease enzyme. ### Claim 3 (depends on 1) A method according to claim 1 wherein the fabric care component comprises a cationic softening compound and/or a silicone softening-compound. ### Claim 4 (depends on 1) A method according to claim 1 wherein the fabric care component is selected from quaternary ammonium compounds. ### Claim 5 (depends on 1) A method according to claim 1 wherein the fabric care component is selected from ester quaternary ammonium compounds, and mixtures thereof. ### Claim 6 (depends on 1) A method according to claim 1 wherein the amount of fabric care component active present in the aqueous liquor is from about 0.005 to about 2 g/l. ### Claim 7 (depends on 1) A method according to claim 1 wherein steps (i) and (ii) are sequential. ### Claim 8 (depends on 1) A method according to claim 1 wherein steps (i) and (ii) are simultaneous. ### Claim 9 (depends on 1) A method according to claim 1 wherein step (i) comprises a rinse step in a laundering process. ### Claim 10 (depends on 1) A method according to claim 1 wherein step (ii) comprises a laundering step. ### Claim 11 (depends on 1) A method according to claim 1 wherein the fabric comprises a cellulosic single or blended textile. ### Claim 12 (depends on 1) A method according to claim 1 wherein the aqueous liquor comprises from about 0.01 ppm to about 1000 ppm of the nuclease enzyme. ### Claim 13 (depends on 1) A method according to claim 1 wherein the enzyme is selected from the nucleases of any of E.C. classes E.C. 3.1.21.x (where x=1, 2, 3, 4, 5, 6, 7, 8, 9), 3.1.22.y (where y=1, 2, 3, 4, 5), E.C. 3.1.30.z (where z=1, 2) or E.C. 3.1.31.1, or mixtures thereof. ### Claim 14 (depends on 1) A method according to claim 1 wherein the enzyme has an amino acid sequence with at least 85% identity with any of the amino acid sequences shown in SEQ ID NO:1, SEQ ID NO2 or SEQ ID NO.3. ### Claim 15 (depends on 1) A method according to claim 1 wherein the enzyme is a microbial ENZYME. ### Claim 16 (depends on 15) A method according to claim 15 wherein the enzyme is a bacterial enzyme. ### Claim 17 (depends on 1) A method according to claim 1 wherein the fabric in step (ii) is contacted with a laundry detergent composition in a wash cycle of an automatic washing machine and wherein the length of the wash cycle is at least about 3 minutes and no more than about 20 minutes. ### Claim 18 (depends on 1) A method according to claim 1, wherein in step (ii) the fabric is contacted with a laundry cleaning and/or treatment composition in a wash cycle of an automatic washing machine and wherein the wash cycle is run at a temperature of between about 10° C. and 30° C.