# US 2020/0399399 A1 · Monoclonal antibody and non-specific reaction inhibitor

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- Number: US 2020/0399399 A1
- Document ID: US20200399399A1
- Status: Published application
- Kind: A1
- Application number: 16/971,452
- Filed: 2019-02-21
- Published: 2020-12-24
- Earliest claimed: 2018-02-21
- Assignees: Tanaka Kikinzoku Kogyo K.K.
- Applicants: Tanaka Kikinzoku Kogyo K.K.
- Inventors: Keita SUZUKI; Hisahiko IWAMOTO
- Web page: https://patentgrep.com/patent/US20200399399A1

## CPC

- C07K 16/4241: {against anti-human or anti-animal Ig}
- C07K 16/4283: {against an allotypic or isotypic determinant on Ig}
- G01N 33/5306: {Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding}
- G01N 33/54388: {based on lateral flow}
- G01N 33/577: involving monoclonal antibodies {binding reaction mechanisms characterised by the use of monoclonal antibodies (G01N33/5302 - G01N33/576 take precedence)}
- C07K 2317/76: Antagonist effect on antigen, e.g. neutralization or inhibition of binding
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

An object of the present invention is to provide a monoclonal antibody capable of sufficiently inhibiting a non-specific reaction caused by a non-specific factor, a non-specific reaction inhibitor containing the monoclonal antibody, and the like. The present invention relates to a monoclonal antibody against dog IgM produced by a hybridoma with accession No. NITE BP-02556.

## Claims

### Claim 1

A monoclonal antibody against dog IgM produced by a hybridoma with accession No. NITE BP-02556.

### Claim 2

A hybridoma with accession No. NITE BP-02556.

### Claim 3 (depends on 1)

A non-specific reaction inhibitor for an immunoassay method, comprising the monoclonal antibody according to claim 1.

### Claim 4 (depends on 3)

The non-specific reaction inhibitor according to claim 3, wherein a content of the monoclonal antibody is 0.5 μg or more and 20 μg or less.

### Claim 5 (depends on 3)

The non-specific reaction inhibitor according to claim 3, wherein the immunoassay method is an immunochromatographic method.

### Claim 6 (depends on 3)

A test strip for immunochromatography, comprising the non-specific reaction inhibitor according to claim 3.

### Claim 7 (depends on 3)

A test kit for immunochromatography, comprising the non-specific reaction inhibitor according to claim 3.

### Claim 8 (depends on 3)

An immunoassay method, comprising performing an immunoreaction in the presence of the non-specific reaction inhibitor according to claim 3 in an immunoassay method for specifically detecting a detection target in a specimen.

### Claim 9 (depends on 8)

The immunoassay method according to claim 8, wherein the immunoassay method is an enzyme immunoassay method, an agglutination method, or an immunochromatographic method.

### Claim 10 (depends on 8)

The immunoassay method according to claim 8, wherein the immunoassay method is an immunochromatographic method.