# US 2020/0399293 A1 · Composition for substrate surface modification and method using the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2020/0399293 A1 - Document ID: US20200399293A1 - Status: Published application - Kind: A1 - Application number: 17/011,470 - Filed: 2020-09-03 - Published: 2020-12-24 - Earliest claimed: 2018-08-07 - Assignees: National Central University - Applicants: National Central University - Inventors: Chen-Han Huang; Wen-Hao Chen - Web page: https://patentgrep.com/patent/US20200399293A1 ## CPC - C07F 7/10: containing nitrogen {having a Si-N linkage} - C12N 11/14: Enzymes or microbial cells immobilised on or in an inorganic carrier - C09D 5/1662: {Synthetic film-forming substance} - C12N 11/02: Enzymes or microbial cells immobilised on or in an organic carrier - C12N 11/06: attached to the carrier via a bridging agent - C12N 11/08: the carrier being a synthetic polymer - C07K 17/06: attached to the carrier via a bridging agent - C07K 17/08: the carrier being a synthetic polymer - C07K 17/14: Peptides being immobilised on, or in, an inorganic carrier - C07F: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM (metal-containing porphyrins C07D487/22) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C09D: COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR (cosmetics A61K; processes for applying liquids or other fluent materials to surfaces, in general, B05D; staining wood B27K5/02; glazes or vitreous enamels C03C; natural resins, French polish, drying-oils, driers, turpentine, per se, C09F; polishing compositions other than French polish, ski waxes C09G; adhesives or use of materials as adhesives C09J; materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12; processes for the electrolytic or electrophoretic production of coatings C25D) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The present invention provides a composition for substrate surface modification and a method using the same, and the composition for substrate surface modification is composed of a compound of the general formula structure shown in formula 1: formula 1, wherein n1 is an integer of 1 to 6, and R is a zwitterionic group. The composition for substrate surface modification uses water as a medium to perform modifying reaction over a substrate surface, and at the same time has biological modification characteristics, and abilities of immobilizing biomolecules and anti-biofouling. ## Claims ### Claim 1 A method for modifying a substrate surface, comprising: preparing a surface modification solution, comprising: providing a 2-(dimethylamino) ethyl trimethoxy silane as a reaction initiator; reacting the reaction initiator with a 4-methyloxetan-2-one to obtain an intermediate product; purifying the intermediate product to obtain a purified intermediate product; adding a triethanol amine and a toluene to react with the purified intermediate product to obtain a final reactant; adding a dimethyl sulfoxide to the final reactant to prepare a standard solution; and diluting the standard solution to form the surface modification solution; providing a substrate to be surface-modified; and coating the surface modification solution on a surface of the substrate to be surface-modified for reaction to modify the surface of the substrate. ### Claim 2 (depends on 1) The method for modifying the substrate surface as claimed in claim 1, wherein the reaction initiator is reacted with the 4-methyloxetan-2-one in an acetonitrile solution. ### Claim 3 (depends on 1) The method for modifying the substrate surface as claimed in claim 1, wherein the step of diluting the standard solution comprises using dichloromethane, ethanol or pure water to dilute. ### Claim 4 (depends on 1) The method for modifying the substrate surface as claimed in claim 1, wherein the substrate is a metal, a polymer or a glass. ### Claim 5 (depends on 1) The method for modifying the substrate surface as claimed in claim 1, wherein after coating the surface modification solution on the surface of the substrate, the method further comprises steps of: activating the surface of the surface-modified substrate using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxy succinimide (NHS); adding a modifying biomolecule to the surface of the substrate for reaction; and performing a deactivating reaction by adding a buffer solution to the surface of the substrate to obtain the surface-modified substrate by the biomolecule.