# US 2020/0392437 A1 · Nucleic acid extraction and purification device and biochemical molecule extraction and purification device > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2020/0392437 A1 - Document ID: US20200392437A1 - Status: Published application - Kind: A1 - Application number: 16/971,652 - Filed: 2019-03-11 - Published: 2020-12-17 - Earliest claimed: 2018-03-13 - Assignees: Wuhan Edebio Technology LLC. - Applicants: Wuhan Edebio Technology LLC. - Inventors: Chuli YI - Web page: https://patentgrep.com/patent/US20200392437A1 ## CPC - C12M 1/12: with sterilisation, filtration or dialysis means - G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 - B01D 29/014: {with curved filtering elements (construction B01D29/035, B01D29/071)} - B01D 29/03: self-supporting - B01D 63/06: Tubular membrane modules - C12M 33/14: {with filters, sieves or membranes} - C12N 15/1017: {by filtration, e.g. using filters, frits, membranes} - B01D 2201/02: Filtering elements having a conical form - G01N 2030/8827: {involving nucleic acids} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract Provided are a nucleic acid extraction and purification device and a biochemical molecule extraction and purification device, belonging to the field of experimental supplies. Both devices have the functionality of a test tube, and thus can hold a liquid to perform preprocessing extraction and purification of nucleic acids or biochemical molecules such as nucleic acids, i.e. achieving a process of cell rupture. Furthermore, both devices can quickly and easily transfer liquid to the extraction membrane, achieving the extraction and purification of nucleic acids or biochemical molecules such as nucleic acids. Furthermore, the two devices do not need to manually fit with a pipettor during the process of liquid transfer or use an automated robotic arm and a mechanical pipettor to complete the process of liquid transfer. ## Claims ### Claim 1 A nucleic acid extraction and purification device, comprising a nucleic acid purification column and a breakable member, wherein a purification column liquid inlet is provided at the top of the nucleic acid purification column, a purification column liquid outlet is provided at the bottom of the nucleic acid purification column, and a nucleic acid extraction membrane is provided in the nucleic acid purification column; and the breakable member is arranged between the purification column liquid inlet and the nucleic acid extraction membrane, and is arranged in the nucleic acid purification column, the breakable member is provided with a groove, and the nucleic acid extraction and purification device further comprises a piercing rod for piercing the breakable member. ### Claim 2 (depends on 1) The nucleic acid extraction and purification device according to claim 1, wherein the groove comprises an annular groove arranged at the center of the breakable member, and a plurality of support legs are provided on a bottom surface of the breakable member on the annular groove. ### Claim 3 (depends on 1) The nucleic acid extraction and purification device according to claim 1, wherein the groove comprises a plurality of strip-shaped grooves arranged along a radius of the breakable member. ### Claim 4 (canceled) ### Claim 5 (depends on 1) The nucleic acid extraction and purification device according to claim 1, wherein the nucleic acid extraction and purification device further comprises a support member, the support member is hermetically installed on an inner wall of the nucleic acid purification column, and the breakable member is fixed to the support member. ### Claim 6 (depends on 5) The nucleic acid extraction and purification device according to claim 5, wherein the support member is a cylinder, or a round sheet with a hole in the middle, or a round basin with a basin bottom facing upward and a hole in the middle of the basin bottom; and the breakable member is sheet-shaped, and is bonded with the support member by hot melting. ### Claim 7 (depends on 5) The nucleic acid extraction and purification device according to claim 5, wherein the support member comprises a round basin with a basin bottom facing upward and a hole in the middle of the basin bottom, and further comprises a snap ring, the snap ring comprises an annular body and an annular snap protrusion arranged on an outer periphery of the annular body, a peripheral wall of the round basin is provided with an annular snapping groove adapted to the annular snapping protrusion, and the snap ring is snapped and installed in the round basin; and the breakable member is sheet-shaped, and is clamped between an upper end surface of the annular body and a lower end surface of the basin bottom. ### Claim 8 (depends on 7) The nucleic acid extraction and purification device according to claim 7, wherein one of the lower end surface of the basin bottom and the upper end surface of the annular body is provided with an annular groove, the other is provided with a protrusion adapted to the annular groove, the breakable member is provided with a bulging portion adapted to the annular groove, and the protrusion is configured to press the bulging portion into the annular groove. ### Claim 9 (depends on 7) The nucleic acid extraction and purification device according to claim 7, wherein an inner edge of the upper end surface of the basin bottom is inclined downward relative to an outer edge of the upper end surface, and an inner edge of the lower end surface of the annular body is inclined upward relative to an outer edge of an outer edge of the lower end surface. ### Claim 10 (depends on 1) The nucleic acid extraction and purification device according to claim 1, wherein the nucleic acid extraction and purification device further comprises a mounting column, the mounting column is sleeved on an inner wall of the nucleic acid purification column, and the breakable member is provided on the mounting column and located above the nucleic acid extraction membrane. ### Claim 11 (depends on 1) The nucleic acid extraction and purification device according to claim 1, wherein the bottom of the piercing rod has a conical structure, a lower part of the piercing rod is sleeved with a stirring sheet, and an outer diameter of the stirring sheet is smaller than an inner diameter of the nucleic acid purification column. ### Claim 12 (depends on 1) The nucleic acid extraction and purification device according to claim 1, wherein the breakable member is a polypropylene breakable member, a polyethylene breakable member, a polytetrafluoroethylene breakable member, an acetate breakable member, a nitrocellulose breakable member, a regenerated fiber breakable member or a special paper breakable member. ### Claim 13 (depends on 1) The nucleic acid extraction and purification device according to claim 1, wherein a ratio of a depth of the groove to a thickness of the breakable member is 100:(101 to 1150). ### Claim 14 (canceled) ### Claim 15 A biochemical molecule extraction and purification device, wherein, the biochemical molecule extraction and purification device comprises an extraction column, an extraction membrane, a liquid temporary blocking mechanism and an execution member, an extraction column inlet is provided at one end of the extraction column, an extraction column outlet is provided at another end of the extraction column, the extraction membrane and the liquid temporary blocking mechanism are both arranged in the extraction column, the liquid temporary blocking mechanism is arranged between the extraction column liquid inlet and the extraction membrane, the liquid temporary blocking mechanism comprises a horizontal supporting portion and at least one detachable portion, the horizontal supporting portion is retained on an inner wall of the extraction column, the horizontal supporting portion is provided with at least one mounting hole, the number of the at least one mounting hole is the same as the number of the at least one detachable portion, one detachable portion is provided in each mounting hole, and the detachable portion is matched with the execution member and is capable of disengaging from the corresponding mounting hole under an action of the execution member. ### Claim 16 (depends on 15) The biochemical molecule extraction and purification device according to claim 15, wherein the detachable portion is a round plug, and the horizontal supporting portion is a cylinder. ### Claim 17 (depends on 15) The biochemical molecule extraction and purification device according to claim 15, wherein the liquid temporary blocking mechanism further comprises an outer longitudinal supporting portion, the outer longitudinal supporting portion is fixed outside the horizontal supporting portion and is perpendicular to the horizontal supporting portion, and the horizontal supporting portion is retained on the inner wall of the extraction column by the outer longitudinal supporting portion. ### Claim 18 (depends on 17) The biochemical molecule extraction and purification device according to claim 17, wherein the detachable portion is sheet-shaped, and a plurality of discontinuous first strip-shaped holes are provided between the detachable portion and the horizontal supporting portion. ### Claim 19 (depends on 18) The biochemical molecule extraction and purification device according to claim 18, wherein the detachable portion is a round sheet, and a plurality of discontinuous second strip-shaped holes are provided on the detachable portion in a radial direction. ### Claim 20 (depends on 19) The biochemical molecule extraction and purification device according to claim 19, wherein the detachable portion comprises a first outer ring and a first inner ring, the first outer ring is radially provided with the plurality of discontinuous second strip-shaped holes, and a plurality of discontinuous third strip-shaped holes are arranged between the first outer ring and the first inner ring. ### Claim 21 (canceled) ### Claim 22 (canceled) ### Claim 23 (depends on 17) The biochemical molecule extraction and purification device according to claim 17, wherein the detachable portion is a round plug, the horizontal supporting portion is a round sheet, the liquid temporary blocking mechanism further comprises an inner longitudinal supporting portion, the inner longitudinal supporting portion is arranged inside the outer longitudinal supporting portion, the inner longitudinal supporting portion is fixed to the inner ring of the horizontal supporting portion and is perpendicular to the horizontal supporting portion, the inner longitudinal supporting portion is a cylinder, and the detachable portion is retained in the inner longitudinal supporting portion. ### Claim 24 (depends on 23) The biochemical molecule extraction and purification device according to claim 23, wherein the inner longitudinal supporting portion is a bottom-sealed cylinder, the detachable portion is retained at an opening of the inner longitudinal supporting portion, and an inner wall of the inner longitudinal supporting portion is provided with at least one window hole.