Method for nk cell transduction

A method for transferring biological material into activated natural killer (NK) cells using a pseudotyped retroviral vector particle or a virus like particle thereof, the method comprising:

• a) obtaining a cell population that comprises NK cells,
• b) activating a plurality of NK cells in the population, and then
• c) contacting the activated NK cells with a plurality of said pseudotyped retroviral vector particle or virus like particle thereof containing said biological material,
• wherein said pseudotyped retroviral vector particle or virus like particle thereof comprises a modified baboon endogenous retrovirus (BaEV) envelope glycoprotein that binds to and fuses with a cell membrane on said activated NK cells, thereby transferring said biological material into the activated NK cells.

A method for treating a human subject in need thereof, comprising:

• 1) obtaining NK cells that contain biological material that is therapeutic to the subject, and
• 2) administering the NK cells to the subject;
• wherein the NK cells are prepared by a process that comprises activating NK cells, and then contacting the activated NK cells with a pseudotyped retroviral vector particle or virus like particle thereof that contains said biological material,
• wherein said pseudotyped retroviral vector particle or virus like particle thereof comprises a modified baboon endogenous retrovirus (BaEV) envelope glycoprotein that binds to and fuses with a cell membrane on said activated NK cells, thereby transferring said biological material into the activated NK cells.