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US 2020/0385704 A1Published application
Expression and secretion system
AssigneeGenentech, Inc.
InventorsDevin TESAR, Xiaocheng CHEN, Mark DENNIS, Isidro HOTZEL
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Abstract
The invention provides an expression and secretion system, and methods of using the same, for the expression and secretion of one fusion protein in prokaryotic cells and a second fusion protein in eukaryotic cells. Also provided herein are nucleic acid molecules, vectors and host cells comprising such vectors and nucleic acid molecules.
Claims20
1-16. (canceled)
A polypeptide comprising, in an N-to-C terminal orientation:
- (i) a signal peptide comprising the amino acid sequence of SEQ ID NO:
- 3;
- (ii) a variable domain, wherein the variable domain comprises an HVR1, an HVR2, and an HVR3;
- and
- (iii) a coat protein or adaptor protein.
2 more elements
A polypeptide comprising, in an N-to-C terminal orientation:
- (i) a signal peptide comprising the amino acid sequence of SEQ ID NO:
- 3, wherein the signal peptide is functional in both a prokaryotic cell and a eukaryotic cell;
- (ii) a VH domain comprising a VH-HVR1, a VH-HVR2, and a VH-VHR3;
- and
- (iii) an Fc region.
2 more elements
An antibody comprising, in an N-to-C terminal orientation:
- (i) a first signal peptide, wherein the first signal peptide is functional in both a prokaryotic cell and a eukaryotic cell;
- (ii) a VL domain;
- (iii) a second signal peptide, wherein the second signal peptide comprises the amino acid sequence of SEQ ID NO:
- 3;
- (iv) a VH domain;
- and
- (v) an Fc region.
4 more elements
Dependencies are inferred from the claim text.