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US 2020/0385691 A1Published application
Method for purificating and crystallizating lpor protein and use thereof
AssigneeZhejiang Shance HeQiShi Bio-Sci & Tech. Co., Ltd.
InventorsQi Cheng, Wenli Sun
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Abstract
Provided are a method for purifying and crystallizing the LPOR protein, by means of which method a protein and crystal with complete structures can be obtained. Also provided are the three-dimensional spatial structure and binding site of the LPOR protein, and the use of the protein.
Claims16
A method for protein crystallization of a light-sensitive protein LPOR, the method comprising:
- providing a purified and concentrated LPOR protein sample, and crystallizing the sample in a crystallization buffer at pH 4.5 to obtain a crystal protein, wherein the crystallization buffer comprises 18% PEG20000 and 0.1M NaAc.
Dependencies are inferred from the claim text.