Methods, systems, computer readable media, and kits for sample identification
AssigneeLife Technologies Corporation
InventorEarl HUBBELL
Life of this patent
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Abstract
A method for sequencing a polynucleotide sample having a barcode sequence, includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined flow ordering; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-tolerant code capable of distinguishing the barcode sequence from other barcode sequences in the presence of one or more errors.
Claims20
A method for sequencing a polynucleotide sample having a barcode sequence, comprising:
- introducing a series of nucleotides to the polynucleotide sample according to a predetermined flow ordering;
- obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample;
- and
- resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-tolerant code capable of distinguishing the barcode sequence from other barcode sequences in the presence of one or more errors.
A system, including:
- a machine-readable memory;
- and
- a processor configured to execute machine-readable instructions, which, when executed by the processor, cause the system to perform a method for sequencing a polynucleotide sample having a barcode sequence, comprising:
- introducing a series of nucleotides to the polynucleotide sample according to a predetermined flow ordering;
- obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample;
- and
- resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-tolerant code capable of distinguishing the barcode sequence from other barcode sequences in the presence of one or more errors.
A set of barcodes for nucleic acid sequencing, comprising:
- a plurality of nucleic acid base sequences, each nucleic acid base sequence being a base space representation of a flowspace representation of a codeword of an error-tolerant sample discrimination code.
Dependencies are inferred from the claim text.