Tissue preparation using nuclease
AssigneeParadigm Diagnostics, Inc.
InventorScott Morris
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Abstract
The disclosed technology relates to a method of analyzing a tissue sample. In one aspect, the method comprises obtaining a formalin-fixed paraffin-embedded (FFPE) tissue sample; contacting the tissue sample with Mung Bean Nuclease to cleave mismatched DNA pairs when isolating DNA from the tissue sample; and performing an analysis on the non-digested DNA. In one embodiment, the tissue sample is obtained from a patient having a refractory disease or a cancer during biopsy, and the analysis comprises performing next-generation DNA sequencing to evaluate genomic DNA mutations. In another embodiment, the method further comprises using a computer to screen for one or more therapies according to the cancer diagnosis; and producing a computer-generated report of possible therapies.
Claims20
A method of analyzing a tissue sample, comprising:
- obtaining a tissue sample;
- isolating DNA from the tissue sample;
- contacting the tissue sample with a nuclease that digests mismatched DNA pairs in the DNA;
- removing the digested DNA;
- and
- performing an analysis on the non-digested DNA.
Dependencies are inferred from the claim text.