Polymerase-nucleotide conjugates for sequencing by trapping
AssigneeElement Biosciences, Inc.
InventorsMatthew Kellinger, Michael Previte, Molly He, Tyler Lopez
Life of this patent
Estimating the term…
Abstract
Compositions and methods comprising the use of polymerase-nucleotide conjugates for nucleic acid sequencing and analysis applications are described.
Claims20
A nucleic acid binding composition comprising a nucleic acid binding moiety and a base-pairing moiety, wherein the base-pairing moiety is connected to the nucleic acid binding moiety via a linker, and wherein the linker has a length effective to allow the base-pairing moiety to pair with a complementary nucleotide in a primed polynucleotide while precluding incorporation of the base-pairing moiety into a 3′ end of the polynucleotide.
A nucleic acid binding composition comprising a mixture of at least one nucleic acid binding moiety and at least four different nucleotides or nucleotide analogs, wherein each nucleic acid binding moiety is tethered to one of the at least four different nucleotides or nucleotide analogs via a linker, and wherein a length of the linker is different for different nucleotides or nucleotide analogs.
Dependencies are inferred from the claim text.