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US 2020/0370109 A1Published application
Novel compositions, methods and kits for real time polymerase chain reaction (pcr)
AssigneeLife Technologies Corporation
InventorsCarole BORNARTH, Michael LAU, Junko STEVENS
Life of this patent
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Abstract
The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.
Claims20
A composition comprising:
- a) a nucleic acid polymerase;
- and
- b) a dual hot start reaction mixture.
A composition comprising:
- a) a thermostable nucleic acid polymerase;
- and
- b) a dual hot start reaction mixture that substantially inhibits the polymerase activity of the polymerase at a temperature less than about 40° C. and wherein the dual hot start reaction mixture does not substantially inhibit the polymerase activity of the polymerase at a temperature greater than about 40° C.
A method for amplifying a target nucleic acid comprising:
- a) contacting the target nucleic acid with a nucleic acid polymerase, a dual hot start reaction mixture, at least one primer and at least one dNTP at a first temperature, thereby forming a reaction composition;
- b) heating the reaction composition to a second temperature;
- and
- c) amplifying the target nucleic acid in the reaction composition.
1 more element
A kit comprising:
- a) a nucleic acid polymerase;
- and
- b) a dual hot start reaction mixture.
Dependencies are inferred from the claim text.