Method for detecting variation in nucleotide sequence on basis of gene panel and device for detecting variation in nucleotide sequence using same

A device for detection of a mutation in a nucleotide sequence, the device comprising a processor operably connected to a communication unit,

• wherein the processor is configured to conduct:
• obtaining a plurality of target genes for one subject sample by using a gene panel including probes for the plurality of target genes through the communication unit;
• collecting multiple replicates of nucleotide sequences including nucleotide sequences matched or unmatched with each of the plurality of target genes by sequencing each of the plurality of target genes in multiple rounds through next generation sequencing;
• matching the multiple replicates of nucleotide sequences with reference nucleotide sequences;
• determining nucleotide sequences unmatched with the reference nucleotide sequences for the plurality of target genes among the multiple replicates of nucleotide sequences;
• and
• determining candidates of nucleotide sequence mutation for the plurality of target genes in the subject sample, based on a probability of mutation for a discordant gene locus of the unmatched nucleotide sequences, where the probability of mutation is calculated by a computational method according to statistical analysis of unmatched nucleotide sequences.