Fluorescent nucleic acid nanostructure-graphene biosensor for nucleic acid detection
AssigneeProgeneer, Inc.
InventorsSoong-Ho UM, Jeonghun KIM, Myung-Ju AHN
Life of this patent
Estimating the term…
Abstract
The present invention relates to a graphene oxide complex having attached thereto a structure composed of single-stranded probes, each comprising a nucleic acid complementary to a target nucleic acid and a fluorescent substance. The graphene oxide complex can detect and quantify multiple biomarkers (target nucleic acids) in real time with high sensitivity at low cost without PCR, and thus can easily diagnose most diseases in which multiple biomarkers are involved, and evaluate the effect of drug use.
Claims15
A composition for nucleic acid detection comprising:
- a fluorescent nucleic acid nanostructure comprising one or more single-stranded probes, each comprising a nucleic acid complementary to a target nucleic acid, a fluorescent substance, and nucleic acids complementary to other single-stranded probes;
- and
- graphene oxide,
- wherein when the target nucleic acid binds to the single-stranded probe to form a double bond, the distance between the probe and the graphene oxide increases and luminescence of the fluorescent substance is detected.
(canceled)
A composition for nucleic acid detection comprising a fluorescent nucleic acid nanostructure and graphene oxide, wherein the fluorescent nucleic acid nanostructure comprises:
- a first single-stranded probe comprising a nucleic acid complementary to a first target nucleic acid and a fluorescent substance;
- a second single-stranded probe comprising a nucleic acid complementary to a second target nucleic acid and a fluorescent substance;
- a third single-stranded probe comprising a nucleic acid complementary to a third target nucleic acid and a fluorescent substance;
- and
- a fourth single-stranded probe consisting of a sequence complementary to the first single-stranded probe, a sequence complementary to the second single-stranded probe, and a sequence complementary to the third single-stranded probe.
A method for nucleic acid detection comprising steps of:
- producing a fluorescent nucleic acid nanostructure by mixing a nucleic acid isolated from a sample with a single-stranded probe comprising a nucleic acid complementary to a target nucleic acid and a fluorescent substance, followed by heat treatment;
- attaching the fluorescent nucleic acid nanostructure to graphene oxide;
- and
- detecting fluorescence by a fluorescence resonance energy transfer phenomenon.
Use of a composition, which comprises a fluorescent nucleic acid nanostructure comprising one or more single-stranded probes, each comprising a nucleic acid complementary to a target nucleic acid, a fluorescent substance, and nucleic acids complementary to other single-stranded probes;
- and graphene oxide, for nucleic acid detection.
Dependencies are inferred from the claim text.