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AssigneeUniversity of Washington
InventorsRaymond J. MONNAT, JR., Blake T. HOVDE, Stefan PELLENZ, Michael PHELPS
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Compositions, targeting reagents, modified cells, nucleic acid molecules, systems, and methods for identifying and selecting genomic safe harbor sites for transgene insertion and other genome engineering applications. These materials and methods can be used to develop desired genome engineering applications, such as transgene insertion and expression or genome modification, that take into account the application-specific needs for safety, functional silence, and accessibility and other factors that vary with a desired application's goals and target population. Representative examples of desired genome engineering applications include, but are not limited to, transgene insertion, such as therapeutic transgene insertion, functional gene editing, gene or chromosomal location-specific structural modification, cell marking, gene activation, and/or gene repression. The desired targeting application may act on the site itself to modify it, for example, or to facilitate insertion of a transgene that, upon expression, could lead to gene activation, repression or further modification.
A method of selecting genomic target sites for a desired genome engineering application, the method comprising:
A method of producing a targeting construct for insertion of a transgene into a genomic site comprising:
Dependencies are inferred from the claim text.