High productivity methods for growing algae
AssigneeTriton Algae Innovations, Inc.
InventorsMiller Tran, Jon Hansen, John Deaton, Xun Wang, Oscar Gonzalez, Michael Mayfield, Stephen Mayfield
Life of this patent
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Abstract
The present disclosure provides for growing algae with an exogenous organic carbon source as the primary carbon source, in light, dark or limited light conditions. Also provided are expression cassettes for expression of a recombinant protein in an algae species grown in dark or limited light conditions.
Claims28
A method for producing a high-density culture of an algae species comprising:
- growing an algae species in the presence of at least one exogenous organic carbon source under aerobic conditions, wherein the algae species is capable of using the organic carbon source as an energy source for growth, and wherein the algae species lacks a chitin cell wall.
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A method for accumulating a recombinant protein from a culture of a Chlamydomonas species comprising:
- (a) providing one or more cells of a recombinant Chlamydomonas species capable of expressing a recombinant protein, wherein the Chlamydomonas species lacks a chitin cell wall;
- (b) growing the one or more cells in the presence of at least one exogenous organic carbon source under aerobic conditions to generate a culture of the recombinant Chlamydomonas species, wherein the Chlamydomonas species uses the organic carbon source as an energy source for growth;
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- (c) harvesting the recombinant protein from the culture.
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An expression cassette comprising an algae 16S promoter fused to a 5′-untranslated region (5′ UTR) and a nucleic acid molecule encoding a recombinant protein, wherein the 5′UTR is selected from the group consisting of psbM, psaA, psaB, psbI, psbK, clpP, rpl4, rps7, rps14, and rps19 5′UTR.
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Dependencies are inferred from the claim text.