Method and apparatus of isolating stromal cells from biological tissue without using enzyme
AssigneeHee Young LEE
InventorsHee Young LEE, Hyun Jin YANG
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Abstract
Provided are a method and an apparatus for isolating stromal cells from biological tissue without using an enzyme, in which spontaneous migration of the stromal cells of the biological tissue is induced to move the stromal cells to the outside of the biological tissue, wherein the induction of the spontaneous migration of the stromal cells is performed in a state in which the biological tissue is attached to an attachment member formed of a material to which biological tissue is attachable, and is performed in a culture medium in which the stromal cells can survive.
Claims29
A method of isolating stromal cells from biological tissue without using an enzyme, the method comprising inducing spontaneous migration of the stromal cells of the biological tissue to move the stromal cells to the outside of the biological tissue, wherein the induction of the spontaneous migration of the stromal cells is performed in a state in which the biological tissue is attached to an attachment member formed of a material to which the biological tissue is attachable, and performed in a culture medium in which the stromal cells are survivable.
A method of isolating stromal cells from biological tissue without using an enzyme, the method comprising:
- (1) finely cutting the biological tissue;
- (2) attaching the finely cut biological tissue to an attachment member in a culture medium, the attachment member being formed of a material to which the biological tissue is attachable;
- (3) inducing spontaneous migration of the stromal cells on the attachment member to move the stromal cells to the outside of the biological tissue;
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- (4) isolating the stromal cells moving to the outside of the biological tissue from the attachment member.
An apparatus for isolating stromal cells from biological tissue without using an enzyme, the apparatus comprising an attachment member configured to move the stromal cells to the outside of the biological tissue by inducing spontaneous migration of the stromal cells of the biological tissue by attaching the biological tissue to the attachment member in a culture medium, wherein the attachment member has a lower or higher average specific gravity than that of the culture medium.
Dependencies are inferred from the claim text.