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US 2020/0369716 A1Published application
Solid-phase synthesis of protein-polymer conjugates on immobilization supports
AssigneeCarnegie Mellon University
InventorsHironobu Murata, Alan J. Russell
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Abstract
Materials and methods for generating protein-polymer conjugates on solid supports are provided herein. The methods can include, for example, reversibly immobilizing a protein on a solid support, modifying the protein by adding polymer subunits, and releasing the protein-polymer conjugate from the solid support.
Claims45
A method for generating a protein-polymer conjugate, comprising:
- attaching a protein of interest to a solid support adapted for coupling to said protein of interest;
- contacting said protein of interest with an initiator;
- and
- coupling a first monomer subunit of a polymer to said protein of interest and, optionally, coupling further monomer subunits to said protein via said first monomer subunit, thus generating said protein-polymer conjugate.
1 more element
A method for generating a protein-polymer conjugate, comprising:
- coupling a protein of interest to a solid support, wherein said solid support comprises DMA-modified agarose beads, and wherein said coupling results in covalent attachment of said protein to said support via a bond between said DMA and an amino group on said protein;
- contacting said protein of interest on said solid support with an ATRP initiator, wherein said ATRP initiator is NETS-Br, and wherein said NHS—Br modifies amino groups on said protein of interest;
- coupling repeating subunits of a polymer to said protein of interest via said ATRP initiator, thus generating said protein-polymer conjugate;
- and
- releasing said protein-polymer conjugate from said solid support by exposing said protein-polymer conjugate on said solid support to a pH between about 3 and about 4, such that said protein-polymer conjugate is released from said solid support.
2 more elements
Dependencies are inferred from the claim text.