Tobamovirus-based virus-like particles and vaccines
AssigneeThe Government of the United States, as represented by the Secretary of the Army
InventorsSheetij Dutta, Adrian Batchelor, Farhat Khan, Mark Langowski
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Abstract
The disclosure generally provides recombinant proteins comprising Tobamovirus capsid proteins and an immunogenic epitope of an antigen of interest. The recombinant protein can be used to assemble an array comprising a plurality of associated recombinant proteins that can enhance the immunogenicity of the epitope and induce and/or enhance an immune response to the antigen. The disclosure also provides compositions, such as vaccines, that include the recombinant protein as well as methods for inducing and/or enhancing an immune response in a mammal.
Claims21
A recombinant protein comprising a Tobamovirus capsid protein and an immunogenic epitope of an antigen of interest.
A method for purifying a recombinant protein comprising a Tobamovirus capsid protein and an immunogenic epitope of an antigen of interest, the method comprising:
- expressing the recombinant protein in a host cell;
- and isolating the recombinant protein.
A method of enhancing immunogenicity of an amino acid antigen, wherein the method comprises:
- expressing a recombinant protein that comprises the amino acid antigen and a Tobamovirus capsid protein;
- isolating the recombinant protein under conditions that allow the recombinant protein to assemble in an array of monomers that forms an interior region and an external region, wherein the amino acid antigen is displayed on the external region of the array of monomers.
Dependencies are inferred from the claim text.