Extracts for the Regeneration of Ligaments
AssigneesUniversity of Pittsburgh—Of the Commonwealth System of Higher Education, Ri.MED Foundation
InventorsRiccardo Luca Gottardi, Benjamin Burke Rothrauff, Rocky Sung Chi Tuan, Shinsuke Kihara
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Abstract
Provided herein is a ligament ECM soluble fraction useful for the growth and regrowth of ligament tissue. Methods of preparation of the soluble fractions and use of the soluble fractions in growth or regrowth of ligament tissue also are provided.
Claims33
A method of inducing growth of ligament or tendon tissue in a patient, comprising, administering to the patient at a site of ligament or tendon damage or defect, a composition comprising a soluble fraction of decellularized, nuclease-digested ligament tissue (soluble fraction) in an amount effective to induce growth of a ligament or tendon in a patient.
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A composition comprising a urea-soluble fraction of decellularized, nuclease-digested ligament tissue prepared according to the method of claim 26, and a pharmaceutically-acceptable excipient.
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A method of preparing a composition for stimulating ligament growth, comprising:
- comminuting ligament tissue;
- decellularizing the tissue in a detergent or emulsifier;
- digesting nucleic acid in the decellularized tissue by treatment of the decellularized tissue with a nuclease;
- dissociating soluble components from insoluble components by exposure of the sample to a protein solubilizing reagent;
- fractionating the composition to separate a soluble fraction from insoluble fibrillar collagen, and collecting the soluble fraction;
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- removing the denaturant from the soluble fraction.
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A method of preparing a ligament structure, comprising applying a soluble fraction of nuclease-digested, decellularized ligament tissue, such as cruciate ligament tissue, such as PCL tissue to a cell growth matrix, optionally an anisotropic cell growth matrix.
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Dependencies are inferred from the claim text.