Vaccine delivery systems using yeast cell wall particles
AssigneeOrbis Health Solutions LLC
InventorThomas E. Wagner
Life of this patent
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Abstract
The present invention generally relates to compositions and methods for delivering a vaccine. The compositions and methods disclosed herein are particularly useful in making prophylactic and therapeutic vaccines.
Claims38
A composition comprising (i) a yeast cell wall particle (YCWP);
- iii) a silicate;
- and (iii) an exogenous biological material loaded within the YCWP, wherein the YCWP is modified by coating with the silicate, wherein the biological material comprises a protein or a fragment thereof, nucleic acid, carbohydrate, antigen or a combination thereof, and wherein the biological material is not a tumor lysate.
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A method for producing an incubated dendritic cell containing a vaccine loaded particle, comprising:
- (i) loading a biological material into the particle to produce a biological material loaded particle;
- (ii) freeze-drying the biological material loaded particle;
- and
- (iii) incubating the biological material loaded particle with a dendritic cell, wherein the biological material comprises a protein or a fragment thereof, nucleic acid, carbohydrate, tumor lysate, or a combination thereof;
- and
- wherein incubating the biological material loaded particle with the dendritic cell causes the dendritic cell to phagocytose the biological material loaded particle.
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A composition comprising a yeast cell wall particle and silicate, wherein the yeast cell wall particle is modified by capping with the silicate.
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A method for preparing a composition comprising a yeast cell wall particle and a silicate, comprising contacting the yeast cell wall particle with the silicate in the presence of ammonia such that the yeast cell wall particle is capped by the silicate.
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A method for producing a cell mixture containing a yeast cell wall particle loaded with a biological material and capped with a silicate, comprising:
- (i) loading a biological material into a yeast cell wall particle to produce a loaded particle;
- (ii) capping the loaded particle with a silicate;
- (iii) freeze-drying the capped, loaded particle;
- and
- (iv) incubating the capped, loaded particle with a cell of monocytic origin
- wherein the incubation causes the cell of monocytic origin to phagocytose the capped, loaded particle.
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A composition for delivering a vaccine, comprising (i) a yeast cell wall particle;
- (ii) a silicate;
- and (iii) an exogenous biological material loaded within the particle, wherein the yeast cell wall particle is modified by coating with the silicate, and wherein the biological material comprises tumor lysate.
Dependencies are inferred from the claim text.