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AssigneeUniversity Health Network
InventorsRalph DaCOSTA, Christopher GIBSON, Kathryn OTTOLINO-PERRY, Nayana Thalanki ANANTHA, Susan Jane DONE, Wey-Liang LEONG, Alexandra M. EASSON
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A method of assessing surgical margins is disclosed. The method includes, subsequent to administration of a compound configured to induce emissions of between about 600 nm and about 660 nm in cancerous tissue cells, positioning a distal end of a handheld, white light and fluorescence-based imaging device adjacent to a surgical margin. The method also includes, with the handheld device, substantially simultaneously exciting and detecting autofluorescence emissions of tissue cells and fluorescence emissions of the induced wavelength in tissue cells of the surgical margin. And, based on a presence or an amount of fluorescence emissions of the induced wavelength detected in the tissue cells of the surgical margin, determining whether the surgical margin is substantially free of at least one of precancerous cells, cancerous cells, and satellite lesions. The compound may be a non-activated, non-targeted compound such as ALA.
A method of assessing surgical margins, comprising:
The method of any one of the preceding claims, further comprising illuminating the tissue cells of the surgical margin with white light and capturing a white light image or video of the surgical margin.
The method of any one of the preceding claims, further comprising transmitting data regarding the white light image or video, the detected autofluorescence emissions of tissue cells, and the fluorescence emissions of the induced porphyrins in tissue cells of the surgical margin from the handheld, white light and fluorescence-based imaging device to a display device.
The method of any one of the preceding claims, wherein the step of substantially simultaneously exciting and detecting is performed between about 15 minutes and about 6 hours after the compound was administered.
The method of any one of the preceding claims, wherein the compound was administered orally, intravenously, via aerosol, via lavage, via immersion, via instillation, and/or topically.
The method of any one of the preceding claims, wherein the compound was administered in a dosage greater than 0 mg/kg and less than 60 mg/kg.
The method of any one of the preceding claims, further comprising identifying a portion of the surgical margin for additional action based on the amount of fluorescence emissions of the induced porphyrins detected in the tissue cells of the surgical margin.
The method of any one of the preceding claims, wherein exciting autofluorescence emissions of tissue cells and fluorescence emissions of the induced porphyrins in tissue cells of the surgical margin includes directing light from at least one excitation light source into a surgical cavity containing the surgical margin, onto an outer surface of an excised tumor or tissue, or onto one or more sections of the excised tumor or tissue.
The method of any of the preceding claims, further comprising filtering emissions from the surgical margin, the emissions being responsive to illumination by at least one excitation light source directed into the surgical cavity containing the surgical margin, onto an outer surface of an excised tumor or tissue, or onto one or more sections of the excised tumor or tissue.
The method of any of claims 54-5457 wherein filtering emissions further comprises permitting passage of emissions having wavelengths from about 700 nm to about 1 micron or from about 700 nm to about 750 nm and about 800 nm to about 1 micron.
The method of any of the preceding claims, wherein detecting autofluorescence emissions of tissue cells and fluorescence emissions of the induced porphyrins in tissue cells of a surgical margin includes detecting filtered emissions from the surgical margin contained in a surgical cavity, an outer surface of an excised tumor or tissue, or a surface of one or more sections of the excised tumor or tissue, the filtered detected emissions being responsive to illumination by at least one excitation light source directed onto the surgical margin.
The method of any of the preceding claims, wherein determining whether the surgical margin is substantially free of cancerous cells includes determining whether the amount of induced poryphins detected in the tissue cells of the surgical margin exceeds a threshold value.
The method of any of the preceding claims, wherein the surgical margin comprises one or more surgical margins.
The method of any one of the preceding claims, wherein fluorescence emissions of the induced porphyrins in tissue cells of the surgical margin is red.
The method of any one of the preceding claims, wherein the cancerous tissue cells comprise breast cancer tissue, brain cancer tissue, colorectal cancer tissue, squamous cell carcinoma tissue, skin cancer tissue, prostate cancer tissue, melanoma tissue, thyroid cancer tissue, ovarian cancer tissue, cancerous lymph node tissue, cervical cancer tissue, lung cancer tissue, pancreatic cancer tissue, head and neck cancer tissue, gastric cancer tissue, liver cancer tissue, or esophageal cancer tissue.
A method of visualizing a tissue of interest in patient, comprising:
A handheld, white light and fluorescence-based imaging device for visualizing at least one of precancerous cells, cancerous cells, and satellite lesions in surgical margins, comprising:
A method of assessing lymph nodes, comprising:
A method of assessing surgical margins, comprising:
A handheld, white light and fluorescence-based imaging device for visualizing at least one of precancerous cells, cancerous cells, and satellite lesions in surgical margins, comprising:
A method of visualizing disease in a patient, comprising:
A method of predicting an amount of fibrosis in a tissue sample, comprising:
A method of correlating tissue types identified in a sample, comprising:
The method of 280, further comprising:
A method of quantifying color contrast in a fluorescence emission of a tissue sample, comprising:
A method of quantifying tissue types in a sample, comprising:
The method of claim 3176, wherein the tissue sample was excited with excitation light emitted by a handheld imaging device in order to produce fluorescent emissions.
Dependencies are inferred from the claim text.