Methods for Vascular Construction and Products Therefrom
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsStephen Dalton, Miranda Kristine Hayworth
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Abstract
The present invention is directed to vascular tissue constructs or vessels and methods for producing vascular tissue constructs or vessels (ie., fabricated blood vessels), including veins, arteries, capillaries and other vascular structures from a biomaterial foundation or scaffold and epicardial progenitor cells (EPCs) which are seeded onto the biomaterial, exposed to a differentiation medium and differentiated into endothelial, cells, smooth muscle cells and pericytes which self-assemble into vascular tissue associated with N the biomaterial foundation or scaffold.
Claims32
A method of producing a vascular tissue containing vessel comprising seeding a tubular biomaterial scaffold with an effective number of migratory mesoderm cells (MesoTs) in association with said scaffold and exposing said MesoTs to a differentiation medium which produces endothelial cells, smooth muscle cells and pericytes from said MesoTs, wherein said endothelial cells, smooth muscle cells and pericytes self-assemble in association with said scaffold to produce said vascular vessel.
A cellularized vascular vessel comprising a tubular biomaterial in association with a population of self-assembled endothelial cells, smooth muscle cells and pericytes which have been differentiated from migratory mesoderm cells (MesoTs).
A kit comprising tubular biomaterial having a diameter of less than 1 mm to 25 cm, a population of MesoTs effective to seed said tubular biomaterial and an effective amount of differentiation medium with instructions for seeding the tubular biomaterial with the MesoTs and differentiating the MesoTs with the differentiation medium into endothelial cells, smooth muscle cells and pericytes which self-assemble into vascular tissue in association with the tubular biomaterial, thus forming a vascular vessel.
A composition comprising between 5×104 and 5×109 MesoT cells in combination with a differentiation medium which excludes Activin A and includes an effective amount of heregulin, IGF, FGF-2 and a VEGF signaling pathway agonist or VEGF receptor agonist.
A method for revascularizing damaged blood vessels and other vascular tissue comprising seeding the surface of damaged blood vessels and/or other vascular tissue with MesoT cells in combination with a differentiation medium for a period of at least 8-10 days to produce endothelial cells, smooth muscle cells and optionally pericytes in said vessels or tissue, which will self-assemble into vascular tissue, thus repairing the damaged blood vessels and/or vascular tissue.
Dependencies are inferred from the claim text.