Method for producing marine algae-derived agarotriose, and use thereof as prebiotic

A food composition comprising:

• one or more substrates selected from the group consisting of agar, agarose, neoagarohexaose, and agarotriose;
• a Bacteroides plebeius strain;
• and
• a Bifidobacterium strain.

A method for producing agarotriose, the method comprising:

• reacting a reaction product with a neoagarobiose hydrolase of SEQ ID NO:
• 2 or 6, wherein the reaction product is obtained by reacting any one substrate of agar, agarose or neoagaroheaose with a β-agarase of SEQ ID NO:
• 1 or 5;
• and
• purifying agarotriose from the resulting product through a size-exclusion column.

A method for treating cancer or an inflammatory disease, the method comprising:

• administering a therapeutically effective amount of a medicine composition to a subject,
• wherein the medicine composition comprises:
• one or more substrates selected from the group consisting of agar, agarose, neoagarohexaose, and agarotriose;
• a Bacteroides plebeius strain;
• and
• a Bifidobacterium strain.