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US 2020/0348299 A1Published application
Methods for detection of flavivirus antibodies
AssigneeThe U.S.A., as represented by the Secretary, Department of Health and Human Services
InventorSurender Khurana
Life of this patent
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Abstract
Isolated peptides that include one or more antigenic sites of Zika virus (ZIKV) and methods of their use and production are disclosed. The peptides can be used, for example, to detect exposure of a subject to a flavivirus infection, such as a ZIKV infection.
Claims21
An isolated peptide comprising an amino acid sequence set forth as SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8 or SEQ ID NO:
- 10, wherein the peptide is no more than 100 amino acids in length, and wherein:
- the peptide is linked to a solid support;
- the peptide is fused to a heterologous protein;
- or
- the peptide is conjugated to a heterologous carrier.
10 more elements
A solid support linked to one or more peptides comprising amino acid sequences selected from SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8 and SEQ ID NO:
- 10, wherein the one or more peptides are no more than 100 amino acids in length.
6 more elements
A method for detecting anti-flavivirus antibodies in a biological sample containing antibodies, comprising:
- contacting the biological sample with one or more peptides comprising amino acid sequences selected from SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8 and SEQ ID NO:
- 10 under conditions sufficient to form an immune complex between the one or more peptides and the antibodies present in the biological sample;
- and
- detecting the presence or absence of the immune complex,
- wherein the presence of the immune complex indicates anti-flavivirus antibodies are present in the sample and the absence of the immune complex indicates anti-flavivirus antibodies are not present in the sample.
10 more elements
Dependencies are inferred from the claim text.