Target Detection with Nanopore

A method of assaying a collection of scaffold-bound fusion molecules, comprising:

• a) contacting a sample containing a plurality of distinct target molecules with a plurality of polymer scaffold-bound fusion molecules, wherein each fusion molecule comprises a distinct target molecule binding domain capable of selectively binding to a distinct target molecule, and further comprising a polymer scaffold binding domain, wherein said polymer scaffold binding domain is permanently bound to said polymer scaffold, and wherein each of the polymer scaffolds comprises a scaffold barcode configured to distinguish between each of the polymer scaffolds;
• b) loading the collection of polymer scaffolds into a device comprising a pore that separates an interior space of the device into two volumes, and configuring the device to pass each of the polymer scaffolds through the pore from one volume to the other volume, wherein the device comprises a sensor configured to identify objects passing through the pore;
• and
• c) determining, with the sensor, whether each distinct target molecule binding domain of each of the fusion molecules is bound to the distinct target molecule upon translocation through the pore, thereby detecting the presence or absence of the plurality of distinct target molecules in the sample.