Psma-based molecular-genetic reporter system
AssigneeThe Johns Hopkins University
InventorsMartin G. Pomper, Mark Castanares, Il Minn, Shawn Lupold
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Abstract
Methods, reporter gene constructs, and kits for using prostate-specific membrane antigen (PSMA) as an imaging reporter to image a variety of cells and tissues are provided.
Claims49
A method for using prostate-specific membrane antigen (PSMA) as an imaging reporter, the method comprising:
- a) introducing a reporter gene construct comprising a PSMA gene operably linked to a transcriptional promoter to a cell;
- b) allowing the cell to express PSMA protein;
- c) adding an imaging probe that can detect the PSMA protein to the cell;
- and
- d) imaging the imaging probe, thereby detecting the PSMA protein.
An adenoviral reporter gene construct comprising a prostate-specific membrane antigen (PSMA) gene operably linked to a cytomegalovirus immediate-early gene (CMV) promoter.
A non-viral, episomal reporter gene construct comprising a prostate-specific membrane antigen (PSMA) gene operably linked to a PEG-Promoter.
Dependencies are inferred from the claim text.