Nucleic acid hybridization methods
AssigneeElement Biosciences, Inc.
InventorsSinan ARSLAN, Molly HE, Michael PREVITE
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Abstract
Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one organic solvent having a dielectric constant in the range of no greater than 115; and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.
Claims33
A method of attaching a target nucleic acid to a surface, comprising
- (a) providing at least one surface bound nucleic acid that is attached to a surface, wherein the surface has a water contact angle of less than 45 degrees;
- and
- (b) contacting the surface bound nucleic acid with a hybridizing composition comprising the target nucleic acid for a period of no more than 20 minutes, wherein the hybridizing composition further comprises:
- (i) at least one organic solvent having a dielectric constant of no greater than 115 at 70° C., and
- (ii) a pH buffer.
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Dependencies are inferred from the claim text.