Transposase compositions, methods of making, and methods of screening

A method of screening a plurality of transposases, comprising:

• (a) contacting a first transposase with a first nucleic acid sample under conditions sufficient to induce transposition of a first oligonucleotide comprising a first end sequence, thereby generating a first transposed nucleic acid sample having a first plurality of insertion sites of the first end sequence;
• (b) contacting a second transposase with a second nucleic acid sample under conditions sufficient to induce transposition of a second oligonucleotide comprising a second end sequence, thereby generating a second transposed nucleic acid sample, the second transposase having an amino acid sequence different from the first transposase by at least one amino acid having a second plurality of insertion sites;
• (c) sequencing at least a portion of the first plurality of insertion sites of the first transposed nucleic acid sample, thereby generating a first set of sequencing reads, each of the first set of sequencing reads comprising one of the insertion sites of the first end sequence;
• (d) sequencing at least a portion of the second plurality of insertion sites of the second transposed nucleic acid sample, thereby generating a second set of sequencing reads, each of the second set of sequencing reads comprising one of the insertion sites of the second end sequence;
• (e) comparing the first set of sequencing reads with the second set of sequencing reads;
• and
• (f) assigning a probability that the second transposase is significantly different from the first transposase based on the step (e) of comparing.