Method for detecting nucleosomes containing nucleotides
AssigneeBelgian Volition SPRL
InventorJacob Vincent Micallef
Life of this patent
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Abstract
The invention relates to a method for detecting and measuring the presence of mono-nucleosomes and oligo-nucleosomes and nucleosomes that contain particular nucleotides and the use of such measurements for the detection and diagnosis of disease. The invention also relates to a method of identifying nucleosome associated nucleotide biomarkers for the detection and diagnosis of disease and to biomarkers identified by said method.
Claims15
A method for detecting cell-free nucleosomes with a 5-methylcytosine or a 5-hydroxymethylcytosine DNA base in a blood, serum or plasma sample which comprises cell-free nucleosomes, comprising:
- contacting the blood, serum or plasma sample with a first binding agent which binds to the cell free nucleosomes and a second binding agent which binds to a 5-methylcytosine or a 5-hydroxymethylcytosine DNA base;
- and
- detecting cell-free nucleosomes with a 5-methylcytosine or 5-hydroxymethylcytosine DNA base that bind to the first binding agent and the second binding agent.
A method to discriminate a blood, serum or plasma sample as from a diseased subject or a healthy subject, comprising:
- providing a blood, serum or plasma sample from the subject;
- contacting the sample with a first binding agent which binds to cell free nucleosomes and with a second binding agent which binds to a 5-methylcytosine or a 5-hydroxymethylcytosine DNA base;
- and
- detecting cell-free nucleosomes with a 5-methylcytosine or 5-hydroxymethylcytosine DNA base that bind to the first binding agent and the second binding agent to discriminate the sample as from a healthy subject or a diseased subject.
A method for detecting or diagnosing a disease status in a subject, comprising:
- (i) detecting or measuring a level or type of cell free nucleosomes containing a particular DNA base, nucleotide or nucleoside in a body fluid of the subject;
- (ii) comparing the detecting or measuring of step (i) to the level or type of cell free nucleosomes containing the particular DNA base, nucleotide or nucleoside in a body fluid of a healthy subject or a control subject;
- and
- (iii) using the difference between the levels or types detected in diseased and control subjects to detect or identify disease status in the subject.
A kit detecting cell-free nucleosomes with a 5-methylcytosine or a 5-hydroxymethylcytosine DNA base in a blood, serum or plasma sample which comprises cell-free nucleosomes, comprising:
- a first binding agent specific for the 5-methylcytosine or a 5-hydroxymethylcytosine DNA base,
- a second binding agent which binds to cell free nucleosomes;
- and
- instructions for use.
Dependencies are inferred from the claim text.