Rapid selective detection of bacteria
AssigneeChapman University
InventorsKamaljit Kaur, Hanieh Hossein-Nejad-Ariani
Life of this patent
Estimating the term…
Abstract
A rapid assay for determining the presence of bacteria in a sample, such as a contaminated food sample, is disclosed. The assay comprises contacting the sample with a bacteria-specific ligand associated with a substrate, wherein bacteria present in the sample bind the ligand; contacting the bound bacteria with a detection agent; detecting the presence of bacteria in the sample by measuring the quantity of detection agent associated with the sample.
Claims44
An assay for determining the presence of Listeria bacteria in a sample comprising:
- contacting the sample with a bacteria-specific ligand associated with a substrate, wherein Listeria bacteria present in the sample bind to the ligand, wherein the bacteria-specific ligand is Leucocin A;
- contacting the bound Listeria bacteria with a detection agent;
- and
- detecting the presence of Listeria bacteria in the sample by measuring the quantity of detection agent associated with the sample.
An assay for determining the presence of Salmonella bacteria in a sample comprising:
- contacting the sample with a bacteria-specific ligand associated with a substrate, wherein Salmonella bacteria present in the sample bind the ligand, wherein the bacteria-specific ligand is microcin N;
- contacting the bound Salmonella bacteria with a detection agent;
- and
- detecting the presence of Salmonella bacteria in the sample by measuring the quantity of detection agent associated with the sample.
A kit for detecting a Listeria species in a sample, the kit comprising:
- a Listeria-specific ligand associated with a substrate, wherein Listeria-specific ligand is Leucocin A;
- gold nanoclusters or reagents to prepare gold nanoclusters;
- and
- instructions for detecting a Listeria species in the sample using the Listeria-specific ligand associated with the substrate.
A kit for detecting a Salmonella species in a sample, the kit comprising:
- a Salmonella-specific ligand associated with a substrate, wherein the Salmonella-specific ligand is monocin N;
- gold nanoclusters or reagents to prepare gold nanoclusters;
- and
- instructions for detecting a Salmonella species in the sample using the Salmonella-specific ligand associated with the substrate.
Dependencies are inferred from the claim text.