Anaplerotic Oil Production In Microbials
AssigneeHeliae Development, LLC
InventorsEneko Ganuza Taberna, Magdalena Amezquita Ernult, Charles Sellers
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Abstract
Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi.
Claims20
A method for producing anaplerotic oil from microalgae, the method comprising:
- adding the microalgae to a culture medium, wherein the microalgae are from the family of thraustochytrids;
- adding propionate to the culture medium resulting in cultured microalgae with enhanced synthesis of saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) odd-chain fatty acid (OFCA) and increased total amounts of tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) present in the microalgae;
- and
- producing an anaplerotic oil from the cultured microalgae, wherein at least five percent of the total fatty acids (TFA) of the anaplerotic oil are saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFA and the saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) make up at least one percent of the cell dry weight (CDW) of the microbial mass.
Dependencies are inferred from the claim text.