Maize Parthenogenetic Haploid-inducing Gene ZmPLA1E and Application Thereof
AssigneeChina Agricultural University
InventorsShaojiang CHEN, Yu ZHONG, Chenxu LIU
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Abstract
The present invention discloses a maize parthenogenetic haploid-inducing gene ZmPLA1E and application thereof. The present invention utilizes the method of exchanged individual plant progeny tests for the first time, and successfully proves that the ZmPLA1E gene can generate and significantly increase the parthenogenetic haploid induction ability in the process of self-crossing or hybridizing as a male parent with other maize materials after the ZmPLA1E gene is mutated in the coding region. The haploid-inducing gene ZmPLA1E of the present invention is important for the cultivation of high-frequency parthenogenetic haploid inducing lines and the application of haploid technology.
Claims13
A method for preparing a plant maternal haploid-inducing line, comprising the following steps:
- silencing or inhibiting the expression and/or activity of the ZmPLA1E gene in the genome of a target plant or knocking out the ZmPLA1E gene to obtain a transgenic plant, i.e., the plant maternal haploid-inducing line.
6-14. (canceled)
A protein, which is a protein of the following a) or b) or c) or d):
- a) a protein with the amino acid sequence set forth in SEQ ID NO:
- 3 or SEQ ID NO:
- 4;
- b) a fusion protein obtained by attaching tag(s) to the N-terminus or/and C-terminus of the protein set forth in SEQ ID NO:
- 3 or SEQ ID NO:
- 4;
- c) a protein having the same function and obtained by subjecting the amino acid sequence set forth in SEQ ID NO:
- 3 or SEQ ID NO:
- 4 to substitution and/or deletion and/or addition of one or more amino acid residues;
- d) a protein having 75% or more of homology with the amino acid sequence set forth in SEQ ID NO:
- 3 or SEQ ID NO:
- 4 and having the same function.
Dependencies are inferred from the claim text.