US 2020/0347393 A1 · MICRO- AND NANONEEDLES FOR PLANT AND OTHER CELL PENETRATION

Abstract

The present invention generally relates to micro- and nanoneedles, e.g., for use in penetrating plant cells, or other cells. In some embodiments, delivery of target biomolecules into cells with protective outer layers (e.g. plant cells or plant pollen), cells in seeds, or cells in tissues may be achieved. These outer layers could be native biological protections (e.g. complex exines for pollens), other types of cells, or general biological materials. In some cases, the needles are attached at an end on a surface. In some cases, pollen or seeds may have substantially thick and tough layers (intine and exine), which may render it more difficult to penetrate such materials, as compared with cell membranes, e.g., in mammalian cells.

Claims75

Dependent claims

A method, comprising:

inserting an array of nanowires into a plant cell, wherein the wires are substantially vertically positioned on a substrate;
and
delivering a biomolecule internally of cells within the plant cell via at least some of the nanowires.

Dependencies are inferred from the claim text.

Facts

Application

16/770,012Filed 7 Dec 2018

Priority

8 Dec 2017

Published

5 Nov 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 15/8207
By physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated: by mechanical means, e.g. microinjection, particle…
C12N 15/89
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation: using microinjection
B82Y 5/00
Nanobiotechnology or nanomedicine
C12N
Microorganisms or enzymes

Micro- and nanoneedles for plant and other cell penetration

Life of this patent

Micro- and nanoneedles for plant and other cell penetration

Life of this patent

Abstract

Claims75