METHOD FOR mRNA STABILIZATION

A method for stabilizing an mRNA, comprising forming a complex consisting of an mRNA encoding a target gene and at least two types of RNA oligomers comprising the following RNA sequence (a) or (b), wherein

• at least the two types of RNA oligomers are capable of hybridizing with each different sequence in the sequence of the mRNA, and
• in the complex, a first sequence of the RNA oligomer hybridizes with an mRNA that is different from an mRNA for a second sequence of the RNA oligomer:
• (a) an RNA sequence comprising a first sequence of 12 to 40 nucleotides complementary to the mRNA sequence, a linker sequence of 0 to 100 nucleotides, and a second sequence that is the same as the first sequence, in this order;
• or
• (b) an RNA sequence comprising a first sequence having an identity of 90% or more to the sequence of 12 to 40 nucleotides complementary to the mRNA sequence and also being capable of hybridizing with the mRNA, a linker sequence of 0 to 100 nucleotides, and a second sequence that is the same as the first sequence, in this order.

A complex consisting of an mRNA encoding a target gene and an RNA oligomer, wherein

• the RNA oligomer is at least one type of RNA oligomer comprising the following RNA sequence (a) or (b), and
• a first sequence of the RNA oligomer hybridizes with an mRNA that is different from an mRNA for a second sequence of the RNA oligomer, so that the complex is formed:
• (a) an RNA sequence comprising a first sequence of 12 to 40 nucleotides complementary to the mRNA sequence, a linker sequence of 0 to 100 nucleotides, and a second sequence that is a sequence of 12 to 40 nucleotides complementary to the mRNA sequence and is different from the first sequence, in this order;
• or
• (b) an RNA sequence comprising a first sequence having an identity of 90% or more to the sequence of 12 to 40 nucleotides complementary to the mRNA sequence and also being capable of hybridizing with the mRNA, a linker sequence of 0 to 100 nucleotides, and a second sequence having an identity of 90% or more to the sequence of 12 to 40 nucleotides complementary to the mRNA sequence, being capable of hybridizing with the mRNA, and being different from the first sequence, in this order.

A method for stabilizing an mRNA, comprising forming a complex consisting of an mRNA encoding a target gene and at least one type of RNA oligomer comprising the following RNA sequence (a) or (b), wherein

• in the complex, a first sequence of the RNA oligomer hybridizes with an mRNA that is different from an mRNA for a second sequence of the RNA oligomer:
• (a) an RNA sequence comprising a first sequence of 12 to 40 nucleotides complementary to the mRNA sequence, a linker sequence of 0 to 100 nucleotides, and a second sequence that is a sequence of 12 to 40 nucleotides complementary to the mRNA sequence and is different from the first sequence, in this order;
• or
• (b) an RNA sequence comprising a first sequence having an identity of 90% or more to the sequence of 12 to 40 nucleotides complementary to the mRNA sequence and also being capable of hybridizing with the mRNA, a linker sequence of 0 to 100 nucleotides, and a second sequence having an identity of 90% or more to the sequence of 12 to 40 nucleotides complementary to the mRNA sequence, being capable of hybridizing with the mRNA, and being different from the first sequence, in this order.